Abstract
The depletion of hydrogen peroxide (H2O2) at a bandwidth of 230 nm was calculated, and the standard method for assessing the H2O2 scavenging activity of bacterial extracts is assessed. Since this approach strains from the interaction of phenolics with heavy uptake in the UV region, a simple and accurate colorimetric assay was developed, whereas bacterial metabolite were added in the context of horseradish peroxidase to the H2O2, phenol, and 4-aminoantipyrine reaction system (HRP). This process generates a chromogen of quinoneimine that can be calculated at 504 nm. The H2O2 scavenged by the plant material is reflected in the decrease in refractive index.
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Chelliah, R., Banan-MwineDaliri, E., Oh, DH. (2022). Screening for Antioxidant Activity: Hydrogen Peroxide Scavenging Assay. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_65
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DOI: https://doi.org/10.1007/978-1-0716-1728-1_65
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