Abstract
Azospirillum is a free-living nitrogen-fixing member of the α-subclass of proteobacteria. Azospirillum is generally found in association with plants and soil and is well known as plant growth promoters. The nitrogen-free semisolid media such as Nfb, RC, FAM, and LGI are employed to isolate Azospirillum genus. This chapter includes isolation protocol for Azospirillum from plant sample (leaf, stem, and root). Rhizosphere soil and its identification methods are discussed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Day JM, Döbereiner J (1976) Physiological aspects of N2-fixation by a spirillum from Digitaria roots. Soil Biol Biochem 8:45–50
Tien TM, Gaskins MH, Hubbell D (1979) Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Pennisetum americanum L.). Appl Environ Microbiol 37:1016–1024
Bashan Y, De-Bashan LE (2010) How the plant growth-promoting bacterium Azospirillum promotes plant growth-a critical assessment. Adv Agron 108:77–136
Magalhães F, Baldani J, Souto S et al (1983) A new acid-tolerant Azospirillum species. An Acad Bras Ciênc 55:417–430
Fred EB, Waksman SA (1928) Laboratory manual of general microbiology. McGraw-Hill Book Co., New York, p 33
Döbereiner J (1995) Isolation and identification of aerobic nitrogen-fixing bacteria from soil and plants. In: Alef K, Nannipieri P (eds) Methods in applied soil microbiology and biochemistry. Academic Press, San Diego, pp 134–141
Lin SY, Shen FT, Young CC (2011) Rapid detection and identifi cation of the free-living nitrogen fi xing genus Azospirillum by 16S rRNA-gene-targeted genus-specific primers. Antonie Van Leeuwenhoek 99:837–844
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Gamit, H.A., Amaresan, N. (2022). Isolation and Identification of Azospirillum. In: Amaresan, N., Patel, P., Amin, D. (eds) Practical Handbook on Agricultural Microbiology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1724-3_4
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1724-3_4
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1723-6
Online ISBN: 978-1-0716-1724-3
eBook Packages: Springer Protocols