Abstract
Observation of protein folding on submillisecond time scales requires specialized ultra-rapid mixers coupled to optical or chemical probes. Here we describe the protocol for employing a microfabricated mixer with a mixing time of 8 μs coupled to a UV confocal microscope. This instrument can detect Trp fluorescence and also excite hydroxyl radicals that label the folding protein which can be detected by mass spectrometry.
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Witalka, D., Lapidus, L.J. (2022). Measurement of Submillisecond Protein Folding Using Trp Fluorescence and Photochemical Oxidation. In: Muñoz, V. (eds) Protein Folding. Methods in Molecular Biology, vol 2376. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1716-8_7
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DOI: https://doi.org/10.1007/978-1-0716-1716-8_7
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1715-1
Online ISBN: 978-1-0716-1716-8
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