Abstract
Small-diameter vascular grafts are considered to be a promising strategy to treat late-stage vascular diseases, one of the largest causes of morbidity and mortality worldwide. However, limited sources of functional vascular cells remain a major obstacle in vascular tissue engineering. Here we describe a novel approach whereby functional vascular cells were obtained by on-site differentiation of human mesenchymal stem cells on a vascular extracellular matrix scaffold under mechanical stimulations in a rotary bioreactor, which has the potential to work as an alternative source for robust implantable artificial vessel grafts.
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References
Unal B, Critchley JA, Capewell S (2003) Missing, mediocre, or merely obsolete? An evaluation of UK data sources for coronary heart disease. J Epidemiol Community Health 57:530–535
Isenberg BC, Williams C, Tranquillo RT (2006) Small-diameter artificial arteries engineered in vitro. Circ Res 98:25–35
Laslett LJ, Alagona P Jr, Clark BA III et al (2012) The worldwide environment of cardiovascular disease: prevalence, diagnosis, therapy, and policy issues a report from the American College of Cardiology. J Am Coll Cardiol 60:S1–S49
Li N, Rickel AP, Sanyour HJ et al (2019) Vessel graft fabricated by the on-site differentiation of human mesenchymal stem cells towards vascular cells on vascular extracellular matrix scaffold under mechanical stimulation in a rotary bioreactor. J Mater Chem B 7:2703–2713
Masuda T, Ukiki M, Yamagishi Y et al (2018) Fabrication of engineered tubular tissue for small blood vessels via three-dimensional cellular assembly and organization ex vivo. J Biotechnol 276:46–53
Nemeno-Guanzon JG, Lee S, Berg JR et al (2012) Trends in tissue engineering for blood vessels. J Biomed Biotechnol 2012:956345
Kurobe H, Maxfield MW, Breuer CK et al (2012) Concise review: tissue-engineered vascular grafts for cardiac surgery: past, present, and future. Stem Cells Transl Med 1:566–571
Pevec WC, Darling RC, Litalien GJ et al (1992) Femoropopliteal reconstruction with knitted, nonvelour Dacron versus expanded polytetrafluoroethylene. J Vasc Surg 16:60–65
Venkatraman S, Boey F, Lao LL (2008) Implanted cardiovascular polymers: natural, synthetic and bio-inspired. Prog Polym Sci 33:853–874
Meiring M, Khemisi M, Laker L et al (2017) Tissue engineered small vessel conduits - the anti-thrombotic effect of re-endothelialization of decellularized baboon arteries: a preliminary experimental study. Med Sci Monit Basic Res 23:344–351
Xu JY, Shi GP (2014) Vascular wall extracellular matrix proteins and vascular diseases. Biochim Biophys Acta 1842:2106–2119
Zhang L, Zhou J, Lu Q et al (2008) A novel small-diameter vascular graft: in vivo behavior of biodegradable three-layered tubular scaffolds. Biotechnol Bioeng 99:1007–1015
Zhao Y, Zhang S, Zhou J et al (2010) The development of a tissue-engineered artery using decellularized scaffold and autologous ovine mesenchymal stem cells. Biomaterials 31:296–307
Catto V, Farè S, Freddi G et al (2014) Vascular tissue engineering: recent advances in small diameter blood vessel regeneration. ISRN Vasc Med 2014:27
Negishi J, Hashimoto Y, Yamashita A et al (2017) Evaluation of small-diameter vascular grafts reconstructed from decellularized aorta sheets. J Biomed Mater Res A 105:1293–1298
Syazwani N, Azhim A, Morimoto Y et al (2015) Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering. J Med Biol Eng 35:258–269
Li N, Sanyour H, Remund T et al (2018) Vascular extracellular matrix and fibroblasts-coculture directed differentiation of human mesenchymal stem cells toward smooth muscle-like cells for vascular tissue engineering. Mater Sci Eng C Mater Biol Appl 93:61–69
Zhang W, Huo Y, Wang X et al (2016) Decellularized ovine arteries as biomatrix scaffold support endothelial of mesenchymal stem cells. Heart Vessel 31:1874–1881
Liu D, Shao X, Zhong Z et al (2018) All-trans retinoic acid in combination with collagen IV induces the differentiation of mouse embryonic stem cells into smooth muscle cells. Int J Clin Exp Med 11:166–172
Ohta R, Niwa A, Taniguchi Y et al (2016) Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells. Sci Rep 6:35680
Henderson K, Sligar AD, Le VP et al (2017) Biomechanical regulation of mesenchymal stem cells for cardiovascular tissue engineering. Adv Healthc Mater 6:1700556
Colazzo F, Alrashed F, Saratchandra P et al (2014) Shear stress and VEGF enhance endothelial differentiation of human adipose-derived stem cells. Growth Factors 32:139–149
Shi Q, Hodara V, Simerly CR et al (2013) Ex vivo reconstitution of arterial endothelium by embryonic stem cell-derived endothelial progenitor cells in baboons. Stem Cells Dev 22:631–642
Acknowledgments
This work was supported in part by the American Heart Association (15SDG25420001) and the South Dakota Board of Regents (UP1600205).
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Li, N., Rickel, A.P., Hong, Z. (2022). On-Site Differentiation of Human Mesenchymal Stem Cells into Vascular Cells on Extracellular Matrix Scaffold Under Mechanical Stimulations for Vascular Tissue Engineering. In: Zhao, F., Leong, K.W. (eds) Vascular Tissue Engineering. Methods in Molecular Biology, vol 2375. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1708-3_4
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DOI: https://doi.org/10.1007/978-1-0716-1708-3_4
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