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Quantification of In Vitro Blood-Brain Barrier Permeability

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Vascular Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2375))

Abstract

In vitro blood-brain barrier (BBB) models have been widely used to simulate in vivo models due to their low cost, feasibility, and repeatability. To serve as a valid substitute, the in vitro BBB should have the similar barrier function as that in vivo. This chapter summarizes the detailed methods for quantifying the barrier function, e.g., the permeability of the BBB to water, ions, and solutes for an in vitro BBB generated on the Transwell filter.

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Acknowledgments

The author would like to thank the funding support from the National Institutes of Health 4UH3TR002151-03.

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Authors and Affiliations

  1. Department of Biomedical Engineering, The City College of the City University of New York, New York, NY, USA

    Bingmei M. Fu

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  1. Bingmei M. Fu
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Correspondence to Bingmei M. Fu .

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Editors and Affiliations

  1. Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA

    Feng Zhao

  2. Department of Biomedical Engineering, Columbia University, New York, NY, USA

    Kam W. Leong

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Fu, B.M. (2022). Quantification of In Vitro Blood-Brain Barrier Permeability. In: Zhao, F., Leong, K.W. (eds) Vascular Tissue Engineering. Methods in Molecular Biology, vol 2375. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1708-3_18

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  • DOI: https://doi.org/10.1007/978-1-0716-1708-3_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1707-6

  • Online ISBN: 978-1-0716-1708-3

  • eBook Packages: Springer Protocols

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