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Rabbit Surgery Protocol for End-to-End and End-to-Side Vascular Graft Anastomosis

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Vascular Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2375))

Abstract

Preclinical testing in animal model is a required stage of vascular device development. Among small animal models, rabbits provide vasculature with relative larger caliber for anastomotic implantation of vascular grafts as preclinical testing before conducting large animal studies. Rabbits have similar hemostatic mechanism with human and can accommodate vascular grafts with various diameters at different locations, and thus provide a valid model to assess small-diameter vascular grafts. This chapter will describe the procedures and materials required to conduct survival surgery in rabbit carotid artery models for implantation of small-diameter tubular grafts with an end-to-side and end-to-end anastomotic technique.

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Acknowledgments

This work was supported by the National Institutes of Health (NIH R01 HL130274-01A1) and NSERC-CREATE, Training in Global Biomedical Technology Research and Innovation at the University of Waterloo [CREATE 509950-2018]. We acknowledge Dr. Filip Konecny, Dr. Kathleen Delaney, and Ms. Jean Flanagan for discussion of surgery methods and Ms. Grace Pohan, Ms. Sabrina Mattiassi, Ms. Rebecca Mac, and the University of Waterloo Central Animal Facility for their technical assistance and discussion.

Author information

Authors and Affiliations

  1. Regenerative Nanomedicine Lab, Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada

    Yuan Yao, YeJin Jeong, Aung Moe Zaw & Evelyn K. F. Yim

  2. Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

    Marek Kukumberg

  3. Tissue Regeneration and Repair Laboratory, Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

    Marek Kukumberg

Authors
  1. Yuan Yao
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  2. YeJin Jeong
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  3. Aung Moe Zaw
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  4. Marek Kukumberg
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  5. Evelyn K. F. Yim
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Corresponding author

Correspondence to Evelyn K. F. Yim .

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA

    Feng Zhao

  2. Department of Biomedical Engineering, Columbia University, New York, NY, USA

    Kam W. Leong

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Yao, Y., Jeong, Y., Zaw, A.M., Kukumberg, M., Yim, E.K.F. (2022). Rabbit Surgery Protocol for End-to-End and End-to-Side Vascular Graft Anastomosis . In: Zhao, F., Leong, K.W. (eds) Vascular Tissue Engineering. Methods in Molecular Biology, vol 2375. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1708-3_15

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  • DOI: https://doi.org/10.1007/978-1-0716-1708-3_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1707-6

  • Online ISBN: 978-1-0716-1708-3

  • eBook Packages: Springer Protocols

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