Abstract
Silk fibroin (SF) is a natural well-known biomaterial that has widely been explored for various tissue engineering applications with great success. Herein, we describe the methodology for fabricating two different types of tubular silk scaffolds aimed for vascular grafting. The first method emphasizes the use of very thin (10–15μm) silk films with unidirectional longitudinal micro-patterns, followed by their sequential rolling, which results in a multilayered tubular graft mimicking native-like cellular composition. The second method describes the fabrication of a bi-layered tubular scaffold comprising of a highly porous inner layer covered with an outer nanofibrous electrospun layer.
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Acknowledgments
We acknowledge the laboratory of Prof. David A. Vorp (University of Pittsburgh, USA) for suggestions during the development of Graft II described in this chapter. P.G. acknowledges the Ministry of Education (MoE), Government of India; the Institute of International Education (IIE), New York; and the United States-India Educational Foundation (USIEF) for research fellowship. B.B.M. acknowledges funding support from the Department of Biotechnology (DBT) and Department of Science and Technology (DST), Government of India. We have filed Indian patents for Graft I (application number 1246/KOL/2015) and Graft II (with or without modifications, application number 201931024432) fabrication methodologies.
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Gupta, P., Mandal, B.B. (2022). Fabrication of Small-Diameter Tubular Grafts for Vascular Tissue Engineering Applications Using Mulberry and Non-mulberry Silk Proteins. In: Zhao, F., Leong, K.W. (eds) Vascular Tissue Engineering. Methods in Molecular Biology, vol 2375. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1708-3_11
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DOI: https://doi.org/10.1007/978-1-0716-1708-3_11
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