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Miniaturized Vascularized Bleeding Model of Hemostasis

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Organ-on-a-Chip

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2373))

Abstract

This chapter describes the development of a poly(dimethylsiloxane)-based microfluidic platform that is able to holistically assess and visualize the entire hemostatic process in vitro. The microfluidic platform includes (1) integration of intact endothelium, (2) physiological flow conditions, (3) controlled mechanical injury to study global hemostatic potential of a patient’s whole blood samples in a microvascular model and for dissecting pathophysiologic mechanisms of diseases.

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References

  1. Sakurai Y et al (2018) A microengineered vascular bleeding model that integrates the principal components of hemostasis. Nat Commun 9:509

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  2. Myers DR et al (2012) Endothelialized microfluidics for studying microvascular interactions in hematologic diseases. J Vis Exp 22:3958

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  3. Occhetta P, Visone R, Rasponi M (2017) High-throughput microfluidic platform for 3D cultures of mesenchymal stem cells. Methods Mol Biol 1612:303–323

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  4. Casquillas GV, Le Berre M, Terriac E, Bertholle F. https://www.elveflow.com/microfluidic/PDMS%20thickness%20VS%20spin%20speed.pdf. Accessed 5 October 2020

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Acknowledgements

Financial support provided by National Institutes of Health, Institute of Heart, Lung, and Blood grant R35HL145000.

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Authors and Affiliations

  1. Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA

    Elaissa Trybus Hardy, Yumiko Sakurai & Wilbur A. Lam

  2. Department of Pediatrics, Division of Pediatric Hematology/Oncology, Emory University School of Medicine, Atlanta, GA, USA

    Elaissa Trybus Hardy, Yumiko Sakurai & Wilbur A. Lam

  3. Aflac Cancer and Blood Disorders Center of Children’s Healthcare of Atlanta, Atlanta, GA, USA

    Elaissa Trybus Hardy & Wilbur A. Lam

Authors
  1. Elaissa Trybus Hardy
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  2. Yumiko Sakurai
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  3. Wilbur A. Lam
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Corresponding author

Correspondence to Wilbur A. Lam .

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Editors and Affiliations

  1. Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milano, Italy

    Marco Rasponi

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Hardy, E.T., Sakurai, Y., Lam, W.A. (2022). Miniaturized Vascularized Bleeding Model of Hemostasis. In: Rasponi, M. (eds) Organ-on-a-Chip. Methods in Molecular Biology, vol 2373. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1693-2_10

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  • DOI: https://doi.org/10.1007/978-1-0716-1693-2_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1692-5

  • Online ISBN: 978-1-0716-1693-2

  • eBook Packages: Springer Protocols

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