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Global Proteome Profiling to Assess Changes in Protein Abundance Using Isobaric Labeling and Liquid Chromatography-Tandem Mass Spectrometry

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Targeted Protein Degradation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2365))

Abstract

Protein degradation is a critical component of all facets of cell biology, and recently methods have been developed to make use of targeted protein degradation as both an investigative tool and a potential therapeutic avenue. Mass spectrometry-based proteomic studies have allowed detailed characterization of changes in protein level and the biology underlying growth, development, and disease. Current methods and instrumentation allow identification and quantitative analysis of thousands of proteins in a single assay. The method described here involves cell lysis and digestion to peptides, labeling peptides with isobaric tagging TMT reagents, basic reversed phase fractionation, and liquid chromatography-tandem mass spectrometry analysis of the enriched peptides.

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Authors and Affiliations

  1. Cell Signaling Technology, INC., Danvers, MA, USA

    Anthony P. Possemato, Kathryn Abell & Matthew P. Stokes

Authors
  1. Anthony P. Possemato
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  2. Kathryn Abell
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  3. Matthew P. Stokes
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Corresponding author

Correspondence to Matthew P. Stokes .

Editor information

Editors and Affiliations

  1. Platform Biology, Arvinas, Inc., New Haven, CT, USA

    Angela M. Cacace

  2. Platform Biology, Arvinas, Inc., New Haven, CT, USA

    Christopher M. Hickey

  3. Platform Biology, Arvinas, Inc., New Haven, CT, USA

    Miklós Békés

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Possemato, A.P., Abell, K., Stokes, M.P. (2021). Global Proteome Profiling to Assess Changes in Protein Abundance Using Isobaric Labeling and Liquid Chromatography-Tandem Mass Spectrometry. In: Cacace, A.M., Hickey, C.M., Békés, M. (eds) Targeted Protein Degradation. Methods in Molecular Biology, vol 2365. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1665-9_16

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  • DOI: https://doi.org/10.1007/978-1-0716-1665-9_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1664-2

  • Online ISBN: 978-1-0716-1665-9

  • eBook Packages: Springer Protocols

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