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Two-Dimensional Gel Electrophoresis Image Analysis

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Proteomics Data Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2361))

Abstract

Gel-based proteomics is still quite widespread due to its high-resolution power; the experimental approach is based on differential analysis, where groups of samples (e.g., control vs diseased) are compared to identify panels of potential biomarkers. However, the reliability of the result of the differential analysis is deeply influenced by 2D-PAGE maps image analysis procedures. The analysis of 2D-PAGE images consists of several steps, such as image preprocessing, spot detection and quantitation, image warping and alignment, spot matching. Several approaches are present in literature, and classical or last-generation commercial software packages exploit different algorithms for each step of the analysis. Here, the most widespread approaches and a comparison of the different strategies are presented.

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Authors and Affiliations

  1. Department of Sciences and Technological Innovation, University of Piemonte Orientale, Alessandria, Italy

    Elisa Robotti, Elisa Calà & Emilio Marengo

  2. Center for Translational Research on Autoimmune and Allergic Diseases, University of Piemonte Orientale, Novara, Italy

    Emilio Marengo

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  1. Elisa Robotti
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  2. Elisa Calà
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  3. Emilio Marengo
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Correspondence to Emilio Marengo .

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Editors and Affiliations

  1. Department of Biotechnology, University of Verona, VERONA, Verona, Italy

    Daniela Cecconi

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Robotti, E., Calà, E., Marengo, E. (2021). Two-Dimensional Gel Electrophoresis Image Analysis. In: Cecconi, D. (eds) Proteomics Data Analysis. Methods in Molecular Biology, vol 2361. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1641-3_1

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  • DOI: https://doi.org/10.1007/978-1-0716-1641-3_1

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