Abstract
In the current chapter, we are detailing the synthesis path of a tumor-targeted, CPP-functionalized chemotherapeutic drug, as well as in vitro validation of the targeting and cell penetrating functionalities of the construct. The design of targeted drug delivery vehicle is based on a new glioma-specific homing peptide that has been conjugated to doxorubicin. Further functionalization with an 18-amino acid cell penetrating peptide pVEC was achieved, a CPP that was chosen because of its high cell penetrating efficacy and low toxicity. The three elements were combined into one drug delivery construct gHope2, and its tumor-homing and cell penetrating activity was demonstrated in human glioma cell line U87.
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References
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Acknowledgments
The authors are supported by the EU project 2014-2020.4.01.15-0013, but this did not bias the current work in any way.
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Kurrikoff, K., Oskolkov, N., Eriste, E., Langel, Ü. (2021). Design and Synthesis of a Peptide-Based Glioma-Targeted Drug Delivery Vector gHope2. In: Hussein, W.M., Stephenson, R.J., Toth, I. (eds) Peptide Conjugation. Methods in Molecular Biology, vol 2355. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1617-8_11
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DOI: https://doi.org/10.1007/978-1-0716-1617-8_11
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