Abstract
Adult human cortical organotypic slice culture is an attractive model system to explore mechanisms of human brain pathology as well as to test drug candidates for treatment of neurodegeneration. Acute studies in human brain slices are limited by the lifetime of the tissue and focus mainly on hippocampus slice preparation. Here we describe the derivation of human organotypic slice cultures of cortical origin, which can be kept in culture for up to 6 weeks. This method enabled us to test the system in coculture with reprogrammed neurons and show its feasibility in neuronal cell integration experiments in human-to-human grafting situation.
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References
Schwarz N, Hedrich UBS, Schwarz H, Harshad PA, Dammeier N, Auffenberg E et al (2017) Human Cerebrospinal fluid promotes long-term neuronal viability and network function in human neocortical organotypic brain slice cultures. Sci Rep 7(1):12249
Eugène E, Cluzeaud F, Cifuentes-Diaz C, Fricker D, Le Duigou C, Clemenceau S et al (2014) An organotypic brain slice preparation from adult patients with temporal lobe epilepsy. J Neurosci Methods 235:234–244
Andersson M, Avaliani N, Svensson A, Wickham J, Pinborg LH, Jespersen B et al (2016) Optogenetic control of human neurons in organotypic brain cultures. Sci Rep 6:24818.SDFSD
Jandová K, Päsler D, Antonio LL, Raue C, Ji S, Njunting M et al (2006) Carbamazepine-resistance in the epileptic dentate gyrus of human hippocampal slices. Brain 129(Pt 12):3290–3306
Ledri M, Sørensen AT, Madsen MG, Christiansen SH, Ledri LN, Cifra A et al (2015) Differential effect of neuropeptides on excitatory synaptic transmission in human epileptic hippocampus. J Neurosci 35(26):9622–9631
Miskinyte G, Devaraju K, Grønning Hansen M, Monni E, Tornero D, Woods NB et al (2017) Direct conversion of human fibroblasts to functional excitatory cortical neurons integrating into human neural networks. Stem Cell Res Ther 8(1):207
Hansen MG, Laterza C, Palma-Tortosa S, Kvist G, Monni E, Tsupykov O et al (2020) Grafted human pluripotent stem cell-derived cortical neurons integrate into adult human cortical neural circuitry. Stem Cells Transl Med 9:1365–1377
Jones RS, da Silva AB, Whittaker RG, Woodhall GL, Cunningham MO (2016) Human brain slices for epilepsy research: pitfalls, solutions and future challenges. J Neurosci Methods 260:221–232
Verwer RW, Sluiter AA, Balesar RA, Baaijen JC, de Witt Hamer PC, Speijer D et al (2015) Injury response of resected human brain tissue in vitro. Brain Pathol 25(4):454–468
Verwer RW, Sluiter AA, Balesar RA, Baayen JC, Speijer D, Idema S et al (2016) Altered loyalties of neuronal markers in cultured slices of resected human brain tissue. Brain Pathol 26(4):523–532
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Kvist, G. (2021). Derivation of Adult Human Cortical Organotypic Slice Cultures for Coculture with Reprogrammed Neuronal Cells. In: Ahlenius, H. (eds) Neural Reprogramming. Methods in Molecular Biology, vol 2352. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1601-7_17
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DOI: https://doi.org/10.1007/978-1-0716-1601-7_17
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-1601-7
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