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Nanoparticles for In Vivo Lifetime Multiplexed Imaging

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Multiplexed Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2350))

Abstract

Lifetime multiplexed imaging refers to the simultaneous labeling of different structures with fluorescent probes that present identical photoluminescence spectra and distinct fluorescence lifetimes. This technique allows extracting quantitative information from multichannel in vivo fluorescence imaging. In vivo lifetime multiplexed imaging requires fluorophores with excitation and emission bands in the near-infrared (NIR) and tunable fluorescence lifetimes, plus an imaging system capable of time-resolved image acquisition and analysis.

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Author information

Authors and Affiliations

  1. Nanomaterials for BioImaging Group, Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS, Madrid, Spain

    Erving Ximendes, Emma Martín Rodríguez & Dirk H. Ortgies

  2. Nanomaterials for BioImaging Group, Departamento de Física de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain

    Erving Ximendes & Dirk H. Ortgies

  3. Fluorescence Imaging Group, Departamento de Física Aplicada, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain

    Emma Martín Rodríguez

  4. School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China

    Meiling Tan & Guanying Chen

  5. ARC Centre of Excellence for Nanoscale Biophotonics, RMIT University, Melbourne, Australia

    Blanca del Rosal

Authors
  1. Erving Ximendes
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  2. Emma Martín Rodríguez
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  3. Dirk H. Ortgies
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  4. Meiling Tan
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  5. Guanying Chen
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  6. Blanca del Rosal
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Corresponding author

Correspondence to Blanca del Rosal .

Editor information

Editors and Affiliations

  1. Department of Cellular Biophysics, Max Planck Institute for Medical Research, Stuttgart, Baden-Württemberg, Germany

    Eli Zamir

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Ximendes, E., Martín Rodríguez, E., Ortgies, D.H., Tan, M., Chen, G., del Rosal, B. (2021). Nanoparticles for In Vivo Lifetime Multiplexed Imaging. In: Zamir, E. (eds) Multiplexed Imaging. Methods in Molecular Biology, vol 2350. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1593-5_15

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  • DOI: https://doi.org/10.1007/978-1-0716-1593-5_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1592-8

  • Online ISBN: 978-1-0716-1593-5

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