Abstract
The fabrication of three-dimensional (3D) cardiac tissue using human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is useful not only for regenerative medicine, but also for drug discovery. Here, we report a bio-3D printer that can fabricate tubular cardiac constructs using only human iPSC-CMs. Protocols to evaluate the contractile force and response to electrical stimulation in the cardiac constructs are described. We confirmed that the constructs can be applied for transplantation or drug response testing. In the near future, we expect that the constructs will be used as alternatives for heart transplantation and in animal experiments for new drug development.
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Acknowledgments
This work was supported by grants from JSPS KAKENHI Grant Number 16K19968, 16K15633 and 18K08763 and the Nakatani Foundation. And this work has received funding by FUJIFILM Corporation in Japan.
Conflicts of Interest
Nakayama is a co-founder and shareholder of Cyfuse Biomedical KK and an inventor/developer designated on patents for the bio-3D printer (Patent title: Method for production of three-dimensional structure of cell; patent number: JP4517125; Patent title: Cell structure production device; patent number; JP5896104). All other authors have declared that no competing interests exist.
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Arai, K., Murata, D., Takao, S., Nakayama, K. (2021). Fabrication of Cardiac Constructs Using Bio-3D Printer. In: Yoshida, Y. (eds) Pluripotent Stem-Cell Derived Cardiomyocytes. Methods in Molecular Biology, vol 2320. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1484-6_6
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DOI: https://doi.org/10.1007/978-1-0716-1484-6_6
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Publisher Name: Humana, New York, NY
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