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Efficient Method to Dissociate Induced Pluripotent Stem Cell-Derived Cardiomyocyte Aggregates into Single Cells

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Pluripotent Stem-Cell Derived Cardiomyocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2320))

Abstract

The human adult heart consists of approximately four billion cardiomyocytes, which do not possess self-renewal abilities. Severe myocardial infarction and dilated cardiomyopathy result in the loss of more than a billion cardiomyocytes. Induced pluripotent stem cells (iPSCs) can differentiate into various types of cells. Due to this ability, these cells could potentially serve as a new resource for cell therapy. Many studies have utilized cardiomyocytes derived from iPSCs for myocardial regeneration therapy. To obtain large number of cardiomyocytes for transplantation, we need to develop effective methods that would allow us to dissociate multiple cardiomyocyte aggregates simultaneously. Here, we describe a method to efficiently dissociate large number of iPSC-derived cardiomyocyte aggregates.

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Author information

Authors and Affiliations

  1. Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Osaka, Japan

    Emiko Ito, Shigeru Miyagawa & Yoshiki Sawa

  2. Cuorips Incorporated, Tokyo, Japan

    Emiko Ito

  3. Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

    Yoshinori Yoshida

Authors
  1. Emiko Ito
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  2. Shigeru Miyagawa
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  3. Yoshinori Yoshida
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  4. Yoshiki Sawa
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Corresponding author

Correspondence to Yoshiki Sawa .

Editor information

Editors and Affiliations

  1. Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

    Yoshinori Yoshida

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Ito, E., Miyagawa, S., Yoshida, Y., Sawa, Y. (2021). Efficient Method to Dissociate Induced Pluripotent Stem Cell-Derived Cardiomyocyte Aggregates into Single Cells. In: Yoshida, Y. (eds) Pluripotent Stem-Cell Derived Cardiomyocytes. Methods in Molecular Biology, vol 2320. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1484-6_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1484-6_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1483-9

  • Online ISBN: 978-1-0716-1484-6

  • eBook Packages: Springer Protocols

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