Abstract
Regenerative medicine using human-induced pluripotent stem cells (hiPSCs) is a promising approach to treat heart failure. However, a large number of cells are required to achieve the desired therapeutic effect. The stirring-type suspension culture method allows a large-scale production of hiPSC-derived cardiomyocytes (more than 1 × 108 cells/100 mL), leading to a stable cell supply. Here, we describe a method to scale-up hiPSC-derived cardiomyocyte production with a high differentiation efficiency.
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Acknowledgment
This was supported by Japan Agency for Medical Research and Development (AMED) under Grant Number JP20bm0204003.
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Sougawa, N., Miyagawa, S., Sawa, Y. (2021). Large-Scale Differentiation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes by Stirring-Type Suspension Culture. In: Yoshida, Y. (eds) Pluripotent Stem-Cell Derived Cardiomyocytes. Methods in Molecular Biology, vol 2320. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1484-6_3
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DOI: https://doi.org/10.1007/978-1-0716-1484-6_3
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1483-9
Online ISBN: 978-1-0716-1484-6
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