Skip to main content
SpringerLink
Log in

Electrophysiological Analysis of hiPSC-Derived Cardiomyocytes Using a Patch-Clamp Technique

  • Protocol
  • First Online:
Pluripotent Stem-Cell Derived Cardiomyocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2320))

Abstract

Electrophysiological analysis of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) using a patch-clamp technique enables the most precise evaluation of electrophysiological properties in single cells. Compared to multielectrode array (MEA) and membrane voltage imaging, patch-clamp recordings offer quantitative measurements of action potentials, and the relevant ionic currents which are essential for the research of disease modeling of inherited arrhythmias, safety pharmacology, and drug discovery using hiPSC-CMs. In this chapter, we describe the detail flow of patch-clamp recordings in hiPSC-CMs.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Hamill OPMA, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch 391:85–100

    Article  CAS  Google Scholar 

  2. Moretti A et al (2010) Patient-specific induced pluripotent stem-cell models for long-QT syndrome. N Engl J Med 363:1397–1409

    Article  CAS  Google Scholar 

  3. Yazawa M, Hsueh B, Jia X, Pasca AM, Bernstein JA, Hallmayer J, Dolmetsch RE (2011) Using induced pluripotent stem cells to investigate cardiac phenotypes in Timothy syndrome. Nature 471:230–234

    Article  CAS  Google Scholar 

  4. Sasaki K et al (2016) Patient-specific human induced pluripotent stem cell model assessed with electrical pacing validates S107 as a potential therapeutic agent for catecholaminergic polymorphic ventricular tachycardia. PLoS One 11:e0164795

    Article  Google Scholar 

  5. Yamamoto Y et al (2017) Allele-specific ablation rescues electrophysiological abnormalities in a human iPS cell model of long-QT syndrome with a CALM2 mutation. Hum Mol Genet 26:1670–1677

    Article  CAS  Google Scholar 

  6. Hayano M et al (2017) Development of a patient-derived induced pluripotent stem cell model for the investigation of SCN5A-D1275N-related cardiac sodium channelopathy. Circ J 81:1783–1791

    Article  CAS  Google Scholar 

  7. Wuriyanghai Y et al (2018) Complex aberrant splicing in the induced pluripotent stem cell-derived cardiomyocytes from a patient with long QT syndrome carrying KCNQ1-A344Aspl mutation. Heart Rhythm 15:1566–1574

    Article  Google Scholar 

  8. Yoshinaga D et al (2019) Phenotype-based high-throughput classification of long QT syndrome subtypes using human induced pluripotent stem cells. Stem Cell Rep 13:394–404

    Article  CAS  Google Scholar 

  9. Bett GC et al (2013) Electronic “expression” of the inward rectifier in cardiocytes derived from human-induced pluripotent stem cells. Heart Rhythm 10:1903–1910

    Article  Google Scholar 

  10. Verkerk AO, Veerman CC, Zegers JG, Mengarelli I, Bezzina CR, Wilders R (2017) Patch-clamp recording from human induced pluripotent stem cell-derived cardiomyocytes: improving action potential characteristics through dynamic clamp. Int J Mol Sci 18(9):1873

    Article  Google Scholar 

  11. Becker N et al (2020) Automated dynamic clamp for simulation of IK1 in human induced pluripotent stem cell-derived cardiomyocytes in real time using Patchliner dynamite(8). Curr Protoc Pharmacol 88:e70

    Article  CAS  Google Scholar 

  12. Ma J et al (2011) High purity human-induced pluripotent stem cell-derived cardiomyocytes: electrophysiological properties of action potentials and ionic currents. Am J Physiol Heart Circ Physiol 301:H2006–H2017

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by JSPS KAKENHI (Grant Number JP17H068806, JP18K15847, JP20K17116 to Y.Y., and JP16K09499, JP19K08538 to T.M.), Fukuda Foundation for Medical Technology (Y.Y), Takeda Science Foundation (Y.Y), research grant from the Japan Agency for Medical Research and Development (T.M.), and Japan Research Foundation for Clinical Pharmacology (T.M.).

Author information

Authors and Affiliations

  1. Department of Bioscience and Genetics, National Cerebral and Cardiovascular Center, Suita, Japan

    Yuta Yamamoto

  2. Department of Cardiovascular Medicine, Kyoto University Graduate School of Medicine, Kyoto, Japan

    Yuta Yamamoto, Sayako Hirose, Yimin Wuriyanghai & Takeru Makiyama

  3. Department of Pediatrics, Kyoto University Graduate School of Medicine, Kyoto, Japan

    Daisuke Yoshinaga

Authors
  1. Yuta Yamamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sayako Hirose
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yimin Wuriyanghai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Daisuke Yoshinaga
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takeru Makiyama
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Takeru Makiyama .

Editor information

Editors and Affiliations

  1. Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

    Yoshinori Yoshida

Rights and permissions

Reprints and permissions

Copyright information

© 2021 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Yamamoto, Y., Hirose, S., Wuriyanghai, Y., Yoshinaga, D., Makiyama, T. (2021). Electrophysiological Analysis of hiPSC-Derived Cardiomyocytes Using a Patch-Clamp Technique. In: Yoshida, Y. (eds) Pluripotent Stem-Cell Derived Cardiomyocytes. Methods in Molecular Biology, vol 2320. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1484-6_13

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-1484-6_13

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1483-9

  • Online ISBN: 978-1-0716-1484-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation