Abstract
FluoVolt, a membrane potential dye, has been used to depict the action potentials of cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CMs) in order to classify the cardiac cell subtype, evaluate long QT syndrome, and conduct cardiotoxic drug-responsive tests. To apply FluoVolt, users must prepare the hiPSC-CMs, assess the dye loadings, and apply the excitation light. Here we describe the steps to measure action potentials from single hiPSC-CMs and hiPSC-CM monolayers using this dye.
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Acknowledgments
We thank Yoko Uematsu and Kaoru Shimizu for their administrative support, and Peter Karagiannis for proofreading the manuscript. This work was supported by a grant from Takeda Pharmaceutical Company Limited, a grant from Leducq foundation (18CVD05), JSPS KAKENHI Grant (17H04176), grants from the Research Center Network for Realization of Regenerative Medicine (JP19bm0104001, JP19bm0204003, and JP19bm0804008), Research on Regulatory Science of Pharmaceuticals and Medical Devices (JP19mk0104117), and Research Project for Practical Applications of Regenerative Medicine (JP19bk0104095) provided by the Japan Agency for Medical Research and Development, and iPS research fund.
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Takaki, T., Yoshida, Y. (2021). Application of FluoVolt Membrane Potential Dye for Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Differentiated via Embryoid Bodies. In: Yoshida, Y. (eds) Pluripotent Stem-Cell Derived Cardiomyocytes. Methods in Molecular Biology, vol 2320. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1484-6_11
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DOI: https://doi.org/10.1007/978-1-0716-1484-6_11
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1483-9
Online ISBN: 978-1-0716-1484-6
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