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Making Cardiomyocytes from Pluripotent Stem Cells

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Pluripotent Stem-Cell Derived Cardiomyocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2320))

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Abstract

The ability to differentiate pluripotent stem cells to cardiomyocyte lineages (PSC-CMs) has opened the door to new disease models and innovative drug and cell therapies for the heart. Nevertheless, further advances in the differentiation protocols are needed to fulfill the promise of PSC-CMs. Obstacles that remain include deriving PSC-CMs with proper electromechanical properties, coalescing them into functional tissue structures, and manipulating the genome to test the impact mutations have on arrhythmias and other heart disorders. This chapter gives a brief consideration of these challenges and outlines current methodologies that offer partial solutions.

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Author information

Authors and Affiliations

  1. Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

    Peter Karagiannis

  2. Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

    Yoshinori Yoshida

Authors
  1. Peter Karagiannis
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  2. Yoshinori Yoshida
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Corresponding author

Correspondence to Yoshinori Yoshida .

Editor information

Editors and Affiliations

  1. Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

    Yoshinori Yoshida

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Karagiannis, P., Yoshida, Y. (2021). Making Cardiomyocytes from Pluripotent Stem Cells. In: Yoshida, Y. (eds) Pluripotent Stem-Cell Derived Cardiomyocytes. Methods in Molecular Biology, vol 2320. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1484-6_1

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  • DOI: https://doi.org/10.1007/978-1-0716-1484-6_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1483-9

  • Online ISBN: 978-1-0716-1484-6

  • eBook Packages: Springer Protocols

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