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MYC Analysis in Cancer and Evolution

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The Myc Gene

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2318))

Abstract

The MYC oncogene was originally identified as a transduced allele (v-myc) in the genome of the highly oncogenic avian retrovirus MC29. The protein product (MYC) of the cellular MYC (c-myc) protooncogene represents the key component of a transcription factor network controlling the expression of a large fraction of all human genes. MYC regulates fundamental cellular processes like growth control, metabolism, proliferation, differentiation, and apoptosis. Mutational deregulation of MYC, leading to increased levels of the MYC protein, is a frequent event in the etiology of human cancers. In this chapter, we describe cell systems and experimental strategies to quantify the oncogenic potential of MYC alleles, to test MYC inhibitors, and to monitor MYC-specific protein–protein interactions that are relevant for the cell transformation process. We also describe experimental procedures to study the evolutionary origin of MYC and to analyze structure, function, and regulation of the ancestral MYC proto-oncogenes.

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Acknowledgments

This work was supported by Austrian Science Fund (FWF) grant P23652 (to K.B.), and by the Tyrolean Science Fund (TWF) grant UNI-0404/688 (to M.H.).

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Authors and Affiliations

  1. Institute of Biochemistry, Center for Molecular Biosciences, University of Innsbruck, Innsbruck, Austria

    Markus Hartl & Klaus Bister

Authors
  1. Markus Hartl
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  2. Klaus Bister
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Corresponding authors

Correspondence to Markus Hartl or Klaus Bister .

Editor information

Editors and Affiliations

  1. Vall d’Hebron Institute of Oncology (VHIO), Vall d’Hebron Barcelona Hospital Campus, Barcelona, Spain

    Laura Soucek

  2. Vall d’Hebron Institute of Oncology (VHIO), Vall d’Hebron Barcelona Hospital Campus, Barcelona, Spain

    Jonathan Whitfield

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Hartl, M., Bister, K. (2021). MYC Analysis in Cancer and Evolution. In: Soucek, L., Whitfield, J. (eds) The Myc Gene. Methods in Molecular Biology, vol 2318. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1476-1_6

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  • DOI: https://doi.org/10.1007/978-1-0716-1476-1_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1475-4

  • Online ISBN: 978-1-0716-1476-1

  • eBook Packages: Springer Protocols

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