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Identifying and Validating MYC:Protein Interactors in Pursuit of Novel Anti-MYC Therapies

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The Myc Gene

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2318))

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Abstract

By identifying MYC protein–protein interactors, we aim to gain a deeper mechanistic understanding of MYC as a regulator of gene transcription and potent oncoprotein. This information can then be used to devise strategies for disrupting critical MYC protein–protein interactions to inhibit MYC-driven tumorigenesis. In this chapter, we discuss four techniques to identify and validate MYC-interacting partners. First, we highlight BioID, a powerful discovery method used to identify high-confidence proximal interactors in living cells. We also discuss bioinformatic prioritization strategies for the BioID-derived MYC-proximal complexes. Next, we discuss how protein interactions can be validated using techniques such as in vivo–in vitro pull-down assays and the proximity ligation assay (PLA). We conclude with an overview of biolayer interferometry (BLI), a quantitative method used to characterize direct interactions between two proteins in vitro. Overall, we highlight the principles of each assay and provide methodology necessary to conduct these experiments and adapt them to the study of interactors of additional proteins of interest.

Brian Raught and Linda Z. Penn are Lead Contacts

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Acknowledgments

We would like to thank the members of the Penn laboratory for helpful discussions and critical review of this chapter. This work was supported by the Canada Research Chairs Program (FRN# 87152, L.Z.P.), Canadian Institutes of Health Research (FRN# 156167, L.Z.P), The Princess Margaret Foundation (B.R., Structure Genomics Consortium (SGC, C.A.), Ontario Graduate Scholarship to D.R. and A.M., and the Peterborough K. M. Hunter Graduate Award (from Peterborough K. M. Hunter Charitable Foundation) to A.M. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

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Resetca, D. et al. (2021). Identifying and Validating MYC:Protein Interactors in Pursuit of Novel Anti-MYC Therapies. In: Soucek, L., Whitfield, J. (eds) The Myc Gene. Methods in Molecular Biology, vol 2318. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1476-1_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1476-1_4

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