Abstract
Nucleic acid polyacrylamide gel electrophoresis (PAGE) has played a critical role in the identification and characterization of viroid RNAs. In addition, double PAGE has been a very efficient tool for the detection of viroids as it is sequence independent and is based on the viroid structure of covalently closed/circular molecules. sPAGE has been widely used for the identification of new viroids as well as a routine detection tool.
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References
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Duran-Vila, N. (2022). Detection of Viroids by sPAGE Gel Electrophoresis. In: Rao, A.L.N., Lavagi-Craddock, I., Vidalakis, G. (eds) Viroids. Methods in Molecular Biology, vol 2316. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1464-8_7
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DOI: https://doi.org/10.1007/978-1-0716-1464-8_7
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