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Metabolomics of Mycobacterium tuberculosis

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Mycobacteria Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2314))

Abstract

Enzymes fuel the biochemical activities of all cells. Their substrates and products thus represent a potential window into the physiologic state of a cell. Metabolomics focuses on the global, or systems-level, study of small molecules in a given biological system and has thus provided an experimental tool with which to study cellular physiology, including the biochemistry within pathogenic microorganisms. While metabolomic studies of Mycobacterium tuberculosis are still in their infancy, recent studies have begun to deliver unique insights into the composition, organization, activity, and regulation of the bacterium’s physiologic network not accessible by other approaches. Here, we outline practical methods for the culture, collection, and analysis of metabolomic samples from M. tuberculosis that emphasize minimally perturbing sample handling, broad and native metabolite recovery, and sensitive, biologically agnostic metabolite detection.

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Acknowledgment

Authors M. Nandakumar and LPS de Carvalho contributed to the previous version of this chapter.

Author information

Authors and Affiliations

  1. Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, New York, NY, USA

    Kyle A. Planck & Kyu Rhee

  2. Department of Pharmacology, Weill Cornell Medicine, New York, NY, USA

    Kyle A. Planck

  3. Department of Microbiology and Immunology, Weill Cornell Medicine, New York, NY, USA

    Kyu Rhee

Authors
  1. Kyle A. Planck
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  2. Kyu Rhee
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Corresponding author

Correspondence to Kyu Rhee .

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Editors and Affiliations

  1. Center for Global Infectious Disease Research, Seattle Children’s Research Institute, Seattle, WA, USA

    Tanya Parish

  2. Center for Global Infectious Disease Research, Seattle Children’s Research Institute, Seattle, WA, USA

    Anuradha Kumar

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Planck, K.A., Rhee, K. (2021). Metabolomics of Mycobacterium tuberculosis . In: Parish, T., Kumar, A. (eds) Mycobacteria Protocols. Methods in Molecular Biology, vol 2314. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1460-0_25

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  • DOI: https://doi.org/10.1007/978-1-0716-1460-0_25

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1459-4

  • Online ISBN: 978-1-0716-1460-0

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