Abstract
Protein A affinity chromatography is widely used for capturing therapeutic antibodies. It offers high binding capacity, selectivity, and resin reusability while delivering high yield and product purity. The Protein A step is ubiquitous in its presence in purification platforms for production of antibody products due to the effective clearance it offers of impurities, high and low molecular weight species (HMW and LMW), host cell proteins (HCP), and DNA. In this chapter, we describe a highly selective Protein A affinity chromatography protocol for purification of monoclonal antibodies.
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Acknowledgments
Financial assistance from the Department of Biotechnology, Ministry of Science and Technology is gratefully acknowledged (number BT/COE/34/SP15097/2015). We are also thankful to GE Healthcare, Sweden, for providing us some of the consumable items used in this investigation.
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Rathore, A.S., Narnaware, S. (2022). Purification of Therapeutic Antibodies by Protein A Affinity Chromatography. In: Houen, G. (eds) Therapeutic Antibodies. Methods in Molecular Biology, vol 2313. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1450-1_9
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DOI: https://doi.org/10.1007/978-1-0716-1450-1_9
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1449-5
Online ISBN: 978-1-0716-1450-1
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