Abstract
Xenorhabdus and Photorhabdus belonging to the family of Enterobacteriaceae are symbionts thriving in commensalism with Steinernema and Heterorhabditis, respectively. Though most of the insect symbionts are endocytobionts, it is hard to cultivate them. Isolation of bacteria from nematodes can be done by release of bacteria from intestine or whole nematodes and through insect hemolymph (G. mellonella). Identification of the symbiotic bacteria helps to verify that the isolates belonged to the genera Xenorhabdus and Photorhabdus. Morphological, biochemical and molecular methodologies are assessed for characterizing the bacterial isolates. Novel bioactive compound of symbiotic bacteria is evaluated by extraction, purification and structural identification. The efficacy, virulence and pathogenicity of symbiotic bacteria are analyzed under laboratory conditions.
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References
Akhurst RJ, Boemare NE (1988) A numerical taxonomic study of the genus Xenorhabdus (Enterobacteriaceae) and proposed elevation of the subspecies of X. nematophilus to species. J Gen Microbiol 134:1835–1845. Printed in Great Britain
Bergey’s Manual of Determinative Bacteriology (2000) Actinomycetales, 9th edn
Sambrook J, Fritschi EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
Bauer AW, Kirby WMM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496
Velikova N, Kavanagh K, Jerry M (2016) Evaluation of Galleria mellonella larvae for studying the virulence of Streptococcus suis. BMC Microbiol 16:291
Mahar AN, Munir M, Elawad S, Gowen SR, Hague NGM (2005) Pathogenicity of bacterium, Xenorhabdus nematophila isolated from entomopathogenic nematode (Steinernema carpocapsae) and its secretion against Galleria mellonella larvae. J Zhejiang Univ Sci B 6(6):457–463
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Sivaramakrishnan, S., Razia, M. (2021). Laboratory Techniques for Symbiotic Bacteria. In: Entomopathogenic Nematodes and Their Symbiotic Bacteria. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-0716-1445-7_9
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DOI: https://doi.org/10.1007/978-1-0716-1445-7_9
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