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Isolation of Primary Human and Rodent Brain Microvascular Endothelial Cells: Culturing, Characterization, and High-Efficiency Transfection

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Neuronal Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2311))

Abstract

Studies of blood–brain barrier (BBB) require developing of a novel and convenient in vitro endothelial cell model. We isolated primary human and rodent brain microvascular endothelial cells and developed methods for culturing, characterization, and high-efficiency transfection of endothelial cells. Here, we describe the improved methods to obtain in vitro human and rodent BBB models to study expression of endogenous and exogenous genes of interest.

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References

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Author information

Authors and Affiliations

  1. Department of Neurology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA

    Nune Darbinian

  2. Department of Pathology, Yale University School of Medicine, New Haven, CT, USA

    Armine Darbinyan

  3. Shriners Hospital Pediatric Research Center, Temple University School of Medicine, Philadelphia, PA, USA

    Nune Darbinian & Nana Merabova

  4. Department of Biology, College of Science & Technology, Temple University, Philadelphia, PA, USA

    Shohreh Amini

Authors
  1. Nune Darbinian
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  2. Armine Darbinyan
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  3. Nana Merabova
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  4. Shohreh Amini
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Corresponding author

Correspondence to Nune Darbinian .

Editor information

Editors and Affiliations

  1. College of Science and Technology, Temple University, Philadelphia, PA, USA

    Shohreh Amini

  2. Department of Neuroscience, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA

    Martyn K. White

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Darbinian, N., Darbinyan, A., Merabova, N., Amini, S. (2021). Isolation of Primary Human and Rodent Brain Microvascular Endothelial Cells: Culturing, Characterization, and High-Efficiency Transfection . In: Amini, S., White, M.K. (eds) Neuronal Cell Culture. Methods in Molecular Biology, vol 2311. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1437-2_15

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  • DOI: https://doi.org/10.1007/978-1-0716-1437-2_15

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1436-5

  • Online ISBN: 978-1-0716-1437-2

  • eBook Packages: Springer Protocols

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