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Evaluation and Quantification of Natural Strigolactones from Root Exudates

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Strigolactones

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2309))

Abstract

Strigolactones (SLs) in the root exudates can be detected by germination assays with root parasitic weed seeds, but precise and accurate evaluation and quantification are possible only by chemical analysis with the liquid chromatography–tandem mass spectrometry (LC-MS/MS). Here we describe methods for root exudate collection, sample preparation, and LC-MS/MS analysis of SLs.

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Acknowledgments

This study was supported by the Japan Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry, the Japan Society for the Promotion of Sciences (KAKENHI 15K07093, 16K07618, 16K18560, 17K07650), and the Japan Science and Technology Agency PRESTO (JPMJPR17QA).

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Authors and Affiliations

  1. Utsunomiya University, Utsunomiya, Japan

    Xiaonan Xie, Takahito Nomura & Koichi Yoneyama

  2. Ehime University, Matsuyama, Japan

    Kaori Yoneyama & Koichi Yoneyama

  3. PRESTO, Japan Science and Technology, Kawaguchi, Japan

    Kaori Yoneyama

Authors
  1. Xiaonan Xie
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  2. Kaori Yoneyama
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  3. Takahito Nomura
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  4. Koichi Yoneyama
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Corresponding author

Correspondence to Koichi Yoneyama .

Editor information

Editors and Affiliations

  1. Department of Chemistry, University of Turin, Turin, Italy

    Cristina Prandi

  2. DISAFA-PlantStressLab, University of Turin, Grugliasco, Italy

    Francesca Cardinale

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Xie, X., Yoneyama, K., Nomura, T., Yoneyama, K. (2021). Evaluation and Quantification of Natural Strigolactones from Root Exudates. In: Prandi, C., Cardinale, F. (eds) Strigolactones. Methods in Molecular Biology, vol 2309. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1429-7_1

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  • DOI: https://doi.org/10.1007/978-1-0716-1429-7_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1428-0

  • Online ISBN: 978-1-0716-1429-7

  • eBook Packages: Springer Protocols

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