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Mass Spectrometry-Based Lipidomics pp 11–37Cite as

High Confidence Shotgun Lipidomics Using Structurally Selective Ion Mobility-Mass Spectrometry

Part of the Methods in Molecular Biology book series (MIMB,volume 2306)

Abstract

Ion mobility (IM) is a gas phase separation strategy that can either supplement or serve as a high-throughput alternative to liquid chromatography (LC) in shotgun lipidomics. Incorporating the IM dimension in untargeted lipidomics workflows can help resolve isomeric lipids, and the collision cross section (CCS) values obtained from the IM measurements can provide an additional molecular descriptor to increase lipid identification confidence. This chapter provides a broad overview of an untargeted ion mobility-mass spectrometry (IM-MS) workflow using a commercial drift tube ion mobility-quadrupole-time-of-flight mass spectrometer (IM-QTOF) for high confidence lipidomics.

Key words

  • Drift tube ion mobility spectrometry
  • Ion mobility spectrometry
  • Collision cross section
  • Tandem MS/MS
  • Lipidomics

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Acknowledgments

This work was supported in part using the resources of the Center for Innovative Technology (CIT) at Vanderbilt University. BSR acknowledges a fellowship from the Vanderbilt Institute for Chemical Biology (VICB). Financial support was provided by the National Institutes of Health (R01GM107978) and the U.S. Environmental Protection Agency (EPA) under Assistance Agreement No. 83573601. This work has not been formally reviewed by the EPA and EPA does not endorse any products or commercial services mentioned in this document. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of the EPA or the U.S. Government.

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Authors and Affiliations

  1. Center for Innovative Technology, Department of Chemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt-Ingram Cancer Center, Vanderbilt University, Nashville, TN, USA

    Bailey S. Rose, Katrina L. Leaptrot, Rachel A. Harris, Stacy D. Sherrod, Jody C. May & John A. McLean

  2. Department of Chemistry, Vanderbilt University, Nashville, TN, USA

    John A. McLean

Authors
  1. Bailey S. Rose
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  2. Katrina L. Leaptrot
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  3. Rachel A. Harris
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  4. Stacy D. Sherrod
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  5. Jody C. May
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  6. John A. McLean
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Editor information

Editors and Affiliations

  1. Mass Spectrometry Resource Division of Endocrinology Metabolism, and Lipid Research Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA

    Ph.D. Fong-Fu Hsu

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Rose, B.S., Leaptrot, K.L., Harris, R.A., Sherrod, S.D., May, J.C., McLean, J.A. (2021). High Confidence Shotgun Lipidomics Using Structurally Selective Ion Mobility-Mass Spectrometry. In: Hsu, FF. (eds) Mass Spectrometry-Based Lipidomics. Methods in Molecular Biology, vol 2306. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1410-5_2

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  • DOI: https://doi.org/10.1007/978-1-0716-1410-5_2

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  • Publisher Name: Humana, New York, NY

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