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Comprehensive Mouse Skin Ceramide Analysis on a Solid-Phase and TLC Separation with High-Resolution Mass Spectrometry Platform

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Mass Spectrometry-Based Lipidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2306))

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Abstract

Lipidomic analyses by mass spectrometry (MS) of epidermal ceramides, a large family of lipids crucial to the permeability barrier of the skin, have been reported previously. To ensure the accuracy of lipid identification, we describe here the isolation of mouse newborn epidermal lipids followed by fractionation with solid-phase extraction columns, and lipidomic analyses by high-resolution MS for structural identification. We also describe here the employment of thin layer chromatography, an old but useful tool, in facilitating the structural characterization of the epidermal lipid species by MS.

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Acknowledgments

This work was supported by NIH grant R01AR049269 to J.H.M., and NIH P30DK020579, P30DK056341, and R24GM136766 grants to Mass Spectrometry Resource of Washington University.

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Authors and Affiliations

  1. Division of Nephrology, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA

    Meei-Hua Lin & Jeffrey H. Miner

  2. Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, USA

    Jeffrey H. Miner

  3. Mass Spectrometry Resource, Division of Endocrinology, Metabolism, and Lipid Research, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA

    Fong-Fu Hsu

Authors
  1. Meei-Hua Lin
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  2. Jeffrey H. Miner
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  3. Fong-Fu Hsu
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Editor information

Editors and Affiliations

  1. Mass Spectrometry Resource Division of Endocrinology Metabolism, and Lipid Research Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA

    Fong-Fu Hsu

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Lin, MH., Miner, J.H., Hsu, FF. (2021). Comprehensive Mouse Skin Ceramide Analysis on a Solid-Phase and TLC Separation with High-Resolution Mass Spectrometry Platform. In: Hsu, FF. (eds) Mass Spectrometry-Based Lipidomics. Methods in Molecular Biology, vol 2306. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1410-5_10

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  • DOI: https://doi.org/10.1007/978-1-0716-1410-5_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1409-9

  • Online ISBN: 978-1-0716-1410-5

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