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Stem-Loop qRT-PCR–Based Quantification of miRNAs

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Small Non-Coding RNAs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2300))

Abstract

Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification method is divided in two steps. First, RNA is reverse transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR. This fast and simple method provides quantitative data with high sensitivity and specificity to study miRNAs and their functions.

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Acknowledgments

This work was supported by the Centre National pour la Recherche Scientifique and the Université de Lorraine.

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Correspondence to Yoann Abel .

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Abel, Y., Rederstorff, M. (2021). Stem-Loop qRT-PCR–Based Quantification of miRNAs. In: Rederstorff, M. (eds) Small Non-Coding RNAs. Methods in Molecular Biology, vol 2300. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1386-3_6

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  • DOI: https://doi.org/10.1007/978-1-0716-1386-3_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1385-6

  • Online ISBN: 978-1-0716-1386-3

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