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Biofilm Formation and Pathogenesis

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Analytical Methodologies for Biofilm Research

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Microbial biofilms are compact surface-attached communities that are usually formed of mixed species wherein microbes thrive together and compete for limited resources. Bacteria growing in biofilms are highly tolerant to antibiotics and recalcitrant to host immune system thus act as an important virulence factor playing a crucial role in infection persistence and pathogenesis. Bacterial biofilms have long been perceived as cause of chronic infections and diseases in humans. However, host–biofilm interactions are not well understood and thus, limiting our knowledge on how biofilms participate in disease pathogenesis. In this chapter, we will discuss underlying molecular mechanisms of biofilm formation and different interactions taking place during establishment of biofilms. Moreover, we will highlight in detail the role of biofilms as a virulence component and its contribution to the disease pathogenesis, focusing on important human infectious diseases and relevant animal models studying the fundamentals of pathogenesis.

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Abbreviations

EPS:

Exopolysaccharides

ECM:

Extracellular matrix

MSCRAMMs:

Microbial surface components recognizing adhesive matrix molecule

PIA:

Polysaccharide intercellular adhesin

AHL:

Acyl-homoserine lactone

AI-2:

Autoinducer-2

HSL:

Homoserine lactone

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  1. Department of Microbiology, Tripura University, Agartala, Tripura, India

    Ankurita Bhowmik, Akshit Malhotra, Sangita Jana & Ashwini Chauhan

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  1. Department of Biotechnology, University of Engineering & Management, Kolkata, West Bengal, India

    Moupriya Nag

  2. Department of Biotechnology, University of Engineering & Management, Kolkata, West Bengal, India

    Dibyajit Lahiri

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Bhowmik, A., Malhotra, A., Jana, S., Chauhan, A. (2021). Biofilm Formation and Pathogenesis. In: Nag, M., Lahiri, D. (eds) Analytical Methodologies for Biofilm Research. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-0716-1378-8_1

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  • DOI: https://doi.org/10.1007/978-1-0716-1378-8_1

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