Abstract
RNA post-transcriptional modifications (PTMs) are progressively gaining relevance in the study of coding-independent functions of RNA. RNA PTMs act as dynamic regulators of several aspects of the RNA physiology, from translation to half-life. Rising interest is supported by the advance of high-throughput techniques enabling the detection of these modifications on a transcriptome-wide scale. To this end, here we illustrate the usefulness of RNA Framework, a comprehensive toolkit for the analysis of RNA PTM mapping experiments, by reanalyzing two published transcriptome-scale datasets of N1-methyladenosine (m1A) and pseudouridine (Ψ) mapping, based on two different experimental strategies.
Key words
- RNA post-transcriptional modifications
- RNA immunoprecipitation
- High-throughput sequencing
- m1A
- N1-methyladenosine
- Pseudouridine
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Acknowledgments
This work was supported by funding from the University of Groningen (Groningen, Netherlands) and the Groningen Biomolecular Sciences and Biotechnology Institute (GBB) to D.I.
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Manfredonia, I., Incarnato, D. (2021). RNA Post-Transcriptional Modification Mapping Data Analysis Using RNA Framework. In: McMahon, M. (eds) RNA Modifications. Methods in Molecular Biology, vol 2298. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1374-0_1
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DOI: https://doi.org/10.1007/978-1-0716-1374-0_1
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