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Detecting ADP-Ribosylation in RNA

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RNA Modifications

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2298))

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Abstract

ADP-ribosylation is a widespread reversible chemical modification of macromolecular targets. Protein ADP-ribosylation has been widely studied and plays a vital role in the regulation of several biological processes. In recent years there has been increasing interest in alternative ADP-ribosylation targets such as nucleic acids—DNA and RNA. Here we report different methods to detect ADP-ribosylation of RNA substrates.

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Acknowledgments

This work was supported by the Wellcome Trust (101794 and 210634), Biotechnology and Biological Sciences Research Council [BB/R007195/1], and Cancer Research United Kingdom [C35050/A22284].

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Authors and Affiliations

  1. Sir William Dunn School of Pathology, University of Oxford, Oxford, UK

    Deeksha Munnur & Ivan Ahel

Authors
  1. Deeksha Munnur
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  2. Ivan Ahel
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Corresponding author

Correspondence to Ivan Ahel .

Editor information

Editors and Affiliations

  1. Department of Urology, University of California, San Francisco, San Francisco, CA, USA

    Mary McMahon

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Munnur, D., Ahel, I. (2021). Detecting ADP-Ribosylation in RNA. In: McMahon, M. (eds) RNA Modifications. Methods in Molecular Biology, vol 2298. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1374-0_15

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  • DOI: https://doi.org/10.1007/978-1-0716-1374-0_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1373-3

  • Online ISBN: 978-1-0716-1374-0

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