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Analyzing the Roles of Rho GTPases in Cancer Cell Adhesion to Endothelial Cells Under Flow Conditions

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Metastasis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2294))

Abstract

Adhesion between cancer cells and endothelial cells, lining the blood vessels, is an important event in tumor progression and metastasis formation. The expression of Rho GTPases is frequently altered in cancers, and they are known to regulate cell migration through their effects on adhesion and cytoskeletal dynamics. Several different types of assays are used to investigate how cancer cells attach to and cross the endothelium. Here, we describe an in vitro technique to study the effects of Rho GTPases on human cancer cell adhesion to endothelial cells under shear stress coupled to live cell imaging.

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Authors and Affiliations

  1. School of Cellular and Molecular Medicine, Biomedical Sciences Building, University Walk, University of Bristol, Bristol, UK

    Camilla Cerutti & Anne J. Ridley

Authors
  1. Camilla Cerutti
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  2. Anne J. Ridley
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Corresponding author

Correspondence to Anne J. Ridley .

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Editors and Affiliations

  1. Translational Oncology of Solid Tumors Experimental and Clinical Research Center, Charité – Universitätsmedizin Berlin and Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

    Ulrike S. Stein

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Cerutti, C., Ridley, A.J. (2021). Analyzing the Roles of Rho GTPases in Cancer Cell Adhesion to Endothelial Cells Under Flow Conditions. In: Stein, U.S. (eds) Metastasis. Methods in Molecular Biology, vol 2294. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1350-4_7

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  • DOI: https://doi.org/10.1007/978-1-0716-1350-4_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1349-8

  • Online ISBN: 978-1-0716-1350-4

  • eBook Packages: Springer Protocols

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