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Rab29 Fast Exchange Mutants: Characterization of a Challenging Rab GTPase

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Rab GTPases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2293))

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Abstract

Rab29 has been implicated in multiple membrane trafficking processes with no described effectors or regulating proteins. Its fast nucleotide exchange rate and inability to bind GDI in cytosol make it a unique and poorly understood Rab. Because the conventional, “GTP-locked” Rab mutation does not have the desired effect in Rab29, we present here the use of a fluorescence-based assay to characterize novel Rab29 mutants (I64T and V156G) that display faster nucleotide exchange rates, allowing for GEF-independent Rab29 activation.

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Authors and Affiliations

  1. Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA

    Rachel C. Gomez, Edmundo G. Vides & Suzanne R. Pfeffer

Authors
  1. Rachel C. Gomez
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  2. Edmundo G. Vides
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  3. Suzanne R. Pfeffer
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Corresponding author

Correspondence to Suzanne R. Pfeffer .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Peggy and Charles Stephenson Cancer Center, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

    Guangpu Li

  2. Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, Chicago, IL, USA

    Nava Segev

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© 2021 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Gomez, R.C., Vides, E.G., Pfeffer, S.R. (2021). Rab29 Fast Exchange Mutants: Characterization of a Challenging Rab GTPase . In: Li, G., Segev, N. (eds) Rab GTPases. Methods in Molecular Biology, vol 2293. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1346-7_2

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  • DOI: https://doi.org/10.1007/978-1-0716-1346-7_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1345-0

  • Online ISBN: 978-1-0716-1346-7

  • eBook Packages: Springer Protocols

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