Abstract
The shed-microspore culture technique is an alternative sub-method combining anther and isolated microspore culture to induce microspore embryogenesis. Recently, its effective use in different types of peppers has drawn attention, because it has a higher embryo yield potential compared to anther culture and is more practical than isolated microspore culture. In this chapter, a stepwise protocol for shed-microspore culture of ornamental pepper is described. This protocol includes the steps of donor plant growth conditions, the choice of suitable flower buds based on DAPI staining of microspores, application of a cold pretreatment to flower buds, surface sterilization of the buds, shed-microspore culture of anthers, stress treatments, regeneration of androgenic in vitro plantlets, their acclimatization and ploidy analysis, and in vivo chromosome doubling of the haploid plants.
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Acknowledgments
This study was carried out under the project of 5120019-TUBITAK-TEYDEB-1505 supported by the Scientific and Technological Research Council of Turkey (TUBITAK) and PEY-ART Co., Antalya, Turkey.
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Ari, E. (2021). Shed-Microspore Culture in Ornamental Peppers for Doubled Haploid Plant Production. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2288. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1335-1_15
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DOI: https://doi.org/10.1007/978-1-0716-1335-1_15
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