Abstract
The development of F1 hybrid vegetable varieties emerges as a result of a great effort, long time, investment, knowledge, and advanced technology. The first stage of hybrid vegetable breeding is obtaining pure lines. It is possible to obtain homozygous parent lines used in the production of hybrid varieties with traditional breeding methods. This period takes 8–10 years, especially in some vegetables which are highly open-pollinated, such as Cucurbita spp. Androgenetic- and/or gynogenetic-based dihaploidization methods provide 100% homozygous pure haploid lines in 1–2 years and save time and effort.
The DH frequency by irradiated pollen technique and anther culture strongly depends on the genotypic response, whereby their practical use in a breeding program is still limited. As a possible alternative technique, gynogenesis (unfertilized ovule/ovarium cultures) switches on to produce haploid plants in some Cucurbita species. In the Cucurbita genus, gynogenesis has been one of the most studied and popular DH techniques and presented remarkable results in recent years.
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References
Whitaker TW, Davis GN (1962) Cucurbits. Botany, cultivation, and utilizatione. Interscience Publishers Inc, New York, USA
Robinson RW, Decker-Walters DS (1996) Crop production science in horticulture 6: Cucurbits CAB InternationalInternational. Wallingford, UK
FAO (2017). FAOSTAT Statistical Databases. www.fao.org
Seymen M, Kurtar ES, Dursun A et al (2019) In sustainable agriculture: assessment of plant growth-promoting rhizobacteria in cucurbitaceous vegetable crops. In: Maheshwari D, Dheeman S (eds) Field crops: sustainable management by PGPR. Sustainable development and biodiversity, vol 23. Springer, Cham
Karaağaç O, Balkaya A (2013) Interspecific hybridization and hybrid seed yield of winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.) lines for rootstock breeding. Sci Hortic 149:9–12
Kurtar ES, Balkaya A (2010) Production of in vitro haploid plants from in situ induced haploid embryos in winter squash (Cucurbita maxima Duchesne ex Lam.) via irradiated pollen. Plant Cell Tiss Org Cult 102:267–277
Kurtar ES, Balkaya A, Ozbakır M et al (2009) Induction of haploid embryo and plant regeneration via irradiated pollen technique in pumpkin (Cucurbita moschata Duchesne ex. Poir). Afr J Biotechnol 8:5944–5951
Chambonnet D, Dumas De Vaulx R (1985) Obtention of embryos and plants from in vitro culture of unfertilized ovules of Cucurbita pepo. Cucurbit Gen Coop Rep 8:66
Metwally EI, Moustafa SA, El-Sawy BI et al (1998b) Production of haploid plants from in vitro culture of unpollinated ovules of Cucurbita pepo L. Plant Cell Tissue Organ Cult 52(3):117–121
Yılmaz Ö (2005) Obtention of haploid plants via ovary culture in summer squash (Cucurbita pepo L.). MSc thesis, Sutcu Imam Univ Ziraat Fak, Kahramanmaras, Turkey
Shalaby TA (2007) Factors affecting haploid induction through in vitro gynogenesis in summer squash (Cucurbita pepo L.). Sci Hortic 115(1):1–6
Domblides EA, Shmykova NA, Zayachkovskaya TV et al (2016) Doubled haploid plant production from in vitro culture of unpollinated ovules of summer squash (Сucurbita pepo L.). Proc of the VII Int Sci and Prac Con, 25 October 2016, p. 352
Rakha MT, Metwally EI, Moustafa SA et al (2012) Evaluation of regenerated strains from six Cucurbita interspecific hybrids obtained through anther and ovule in vitro cultures. Aust J Crop Sci 6(1):23–30
Min ZY, Li H, Zou T et al (2016) Studies of in vitro culture and plant regeneration of unfertilized ovary of pumpkin. Chin Bull Bot 51(1):74–80
Kurtar ES, Balkaya A, Ozbakir Ozer M (2018) Production of callus mediated gynogenic haploids in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.). Czech J Genet Plant Breed 54(1):9–16
Zou T, Song H, Chu X et al (2020) Efficient induction of gynogenesis through unfertilized ovary culture with winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.). Sci Hortic 264:109152
Kurtar ES, Balkaya A, Kandemir D (2016) Evaluation of haploidization efficiency in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.) through anther culture. Plant Cell Tiss Org Cult 127:497–511
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15(3):473–497
Sari N (1994) Effect of genotype and season on the obtention of haploid plants by irradiated pollen in watermelon and alternatives to the irradiation. Ph.D. thesis, University of Cukurova, Adana, Turkey
Dore C (1986) Evaluation du niveau de ploidie des plantes d’une population de choux de Bruxelles (Brassica oleracea L. ssp. gemmifera) d’origine pollinique. Agronomie 6:797–801
Kwack SN, Fujieda K (1988) Somatic embryogenesis in cultured unfertilized ovules of Cucurbita moschata. J Jpn Soc Hortic Sci 57:34–42
Xie B, Wang XF, Fan ZC (2006) Improved conditions of in vitro culture of unpollinated ovules and production of embryonary sac plants in summer squash (Cucurbita pepo L.). Sci Agric Sin 39(1):132–138
Sun SR, Zhai QH, Hu JB et al (2009) Effects of several physiological factors on embryo formation in unpollinated ovary culture of pumpkin (Cucurbita moschata Duch. ex Poiret). Plant Physiol Commun 45(10):977–980
Kara B, Sarı N (2019) Effects of spermidine and putrescine applications on haploid plant obtention via ovule culture in Sakız and dark green squashes (Cucurbita pepo L.) Turk. J Agric Res 6(2):206–213
Alan AR, Mutschler MA, Brant A et al (2003) Production of gynogenic plants from hybrids of Allium cepa L. and A. roylei Stearn. Plant Sci 165:1201–1211
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Kurtar, E.S., Seymen, M. (2021). Gynogenesis in Cucurbita Species. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2289. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1331-3_8
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DOI: https://doi.org/10.1007/978-1-0716-1331-3_8
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