Abstract
Haploid production is of great importance in plant breeding programs. Doubled haploid technology accelerates the generation of inbred lines with homozygosity in all loci in a single year. Haploids can be induced in vitro via cultivating the haploid gametes or in vivo through inter- and intraspecific hybridization. Haploid induction through centromere engineering is a novel system that is theoretically applicable to many plant species. The present review chapter discusses the proposed molecular mechanisms of selective chromosome elimination in early embryogenesis and the effects of kinetochore component modifications on proper chromosome segregation. Finally, the advantages and limitations of the CENH3-mediated haploidization approach and its applications are highlighted.
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The author is very grateful to Dr. Ali Mohammad Banaei-Moghaddam, Institute of Biochemistry and Biophysics (IBB), University of Tehran, for valuable suggestions and critical reading of the manuscript.
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Karimi-Ashtiyani, R. (2021). Centromere Engineering as an Emerging Tool for Haploid Plant Production: Advances and Challenges. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2289. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1331-3_1
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