Abstract
This chapter describes the methods for pre-analytical processing and biobanking of cerebrospinal fluid (CSF) samples. There is increasing knowledge that pre-analytical procedures and storage conditions can influence biomarker results, which is relevant to all kinds of fluids and thus also biomarkers measured in CSF. The pre-analytical phase includes patient-related factors, such as fasting, as well as processing factors, such as delay between collection and processing, type of tube, or number of transfers of the fluid. As for the processing factors, a distinction can be made whether analysis is performed for routine care or research purposes, which mostly affects the storage time and storage temperatures. For example, routine analysis usually implies storage of days to weeks at −20 °C or 4 °C, while research analysis usually implies storage for years at −80 °C. The presented protocol is based on previously published protocols for CSF collection and long-term biobanking in the context of biomarker development or research, with adaptations based on experimental evidence collected over time.
The chapter therefore provides two different protocols pertaining to these two different purposes.
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References
Plebani M (2006) Errors in clinical laboratories or errors in laboratory medicine? Clin Chem Lab Med 44(6):750–759. https://doi.org/10.1515/CCLM.2006.123
Lippi G, Guidi GC, Mattiuzzi C, Plebani M (2006) Preanalytical variability: the dark side of the moon in laboratory testing. Clin Chem Lab Med 44(4):358–365. https://doi.org/10.1515/CCLM.2006.073
Plebani M, Sciacovelli L, Aita A (2017) Quality indicators for the total testing process. Clin Lab Med 37(1):187–205. https://doi.org/10.1016/j.cll.2016.09.015
Vos SJB, Visser PJ, Verhey F et al (2014) Variability of CSF Alzheimer’s disease biomarkers: implications for clinical practice. PLoS One 9(6):e100784. https://doi.org/10.1371/journal.pone.0100784
del Campo M, Mollenhauer B, Bertolotto A et al (2012) Recommendations to standardize preanalytical confounding factors in Alzheimer’s and Parkinson’s disease cerebrospinal fluid biomarkers: an update. Biomark Med 6(4):419–430. https://doi.org/10.2217/bmm.12.46
Teunissen CE, Petzold A, Bennett JL et al (2009) A consensus protocol for the standardization of cerebrospinal fluid collection and biobanking. Neurology 73(22):1914–1922. https://doi.org/10.1212/WNL.0b013e3181c47cc2
Teunissen CE, Tumani H, Bennett JL et al (2011) Consensus guidelines for CSF and blood biobanking for CNS biomarker studies. Mult Scler Int 2011:246412. https://doi.org/10.1155/2011/246412
Vanderstichele H, Bibl M, Engelborghs S et al (2012) Standardization of preanalytical aspects of cerebrospinal fluid biomarker testing for Alzheimer’s disease diagnosis: a consensus paper from the Alzheimer’s biomarkers standardization initiative. Alzheimers Dement 8(1):65–73. https://doi.org/10.1016/j.jalz.2011.07.004
Vanderstichele HMJ, Janelidze S, Demeyer L et al (2016) Optimized standard operating procedures for the analysis of cerebrospinal fluid Aβ42 and the ratios of Aβ isoforms using low protein binding tubes. J Alzheimers Dis 53:1121–1132. https://doi.org/10.3233/JAD-160286
Willemse EAJ, Teunissen CE (2015) Biobanking of cerebrospinal fluid for biomarker analysis in neurological diseases. In: Karimi-Busheri F (ed) Biobanking in the 21st century, advances in experimental medicine and biology. Springer International Publishing, Basel, pp 79–92. https://doi.org/10.1007/978-3-319-20579-3
Hok-A-Hin YS, Willemse EAJ, Teunissen CE, Del Campo M (2019) Guidelines for CSF processing and biobanking: impact on the identification and development of optimal CSF protein biomarkers. In: Humana, New York, NY, pp 27–50. https://doi.org/10.1007/978-1-4939-9706-0_2
Willemse EAJ, Teunissen CE (2015) Importance of pre-analytical stability for CSF biomarker testing. https://doi.org/10.1007/978-3-319-01225-4_5
Teunissen CE, Verheul C, Willemse EAJ (2017) The use of cerebrospinal fluid in biomarker studies. In: Deisenhammer F, Teunissen CE, Tumani H (eds) Handbook of clinical neurology, cerebrospinal fluid in neurologic disorders. Elsevier, pp 3–20. https://doi.org/10.1016/B978-0-12-804279-3.00001-0
Willemse E, van Uffelen K, Brix B, Engelborghs S, Vanderstichele H, Teunissen C (2017) How to handle adsorption of cerebrospinal fluid amyloid-β (1–42) in laboratory practice? Identifying problematic handlings and resolving the issue by use of the Aβ 42/Aβ 40 ratio. Alzheimers Dement 13(8):885–892. https://doi.org/10.1016/j.jalz.2017.01.010
Willemse EAJ, Koel-simmelink MJA, Durieux-lu S, Van Der Flier WM, Teunissen CE (2012) Standard biobanking conditions are sufficient to prevent biofluid sample evaporation. 2013
Willemse EAJ, Vermeiren Y, Garcia-Ayllon M-S et al (2019) Pre-analytical stability of novel cerebrospinal fluid biomarkers. Clin Chim Acta 497:204–211. https://doi.org/10.1016/J.CCA.2019.07.024
Pitt K, Betsou F (2012) The ISBER best practices self assessment tool (SAT): lessons learned after three years of collecting responses. Biopreserv Biobank 10(6):548–549. https://doi.org/10.1089/bio.2012.1064
Gaignaux A, Ashton G, Coppola D et al (2016) A biospecimen proficiency testing program for biobank accreditation: four years of experience. Biopreserv Biobank 14(5):429–439. https://doi.org/10.1089/bio.2015.0108
Lewczuk P, Gaignaux A, Kofanova O et al (2018) Interlaboratory proficiency processing scheme in CSF aliquoting: implementation and assessment based on biomarkers of Alzheimer’s disease. Alzheimers Res Ther 10(1):87. https://doi.org/10.1186/s13195-018-0418-3
Willemse EAJ, van Uffelen KWJ, van der Flier WM, Teunissen CE (2017) Effect of long-term storage in biobanks on cerebrospinal fluid biomarker Aβ 1-42 , T-tau, and P-tau values. Alzheimers Dement Diagn Assess Dis Monit 8:45–50. https://doi.org/10.1016/j.dadm.2017.03.005
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Teunissen, C.E., Willemse, E. (2021). Pre-Analytical Processing and Biobanking Protocol for CSF Samples. In: Teunissen, C.E., Zetterberg, H. (eds) Cerebrospinal Fluid Biomarkers. Neuromethods, vol 168. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1319-1_8
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DOI: https://doi.org/10.1007/978-1-0716-1319-1_8
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