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Quantification of the Neurofilament Light Chain Protein by Single Molecule Array (Simoa) Assay

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Cerebrospinal Fluid Biomarkers

Part of the book series: Neuromethods ((NM,volume 168))

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Abstract

Neurofilaments are cytoskeletal proteins specific for neurons. They are released upon neuronal injury and are increasingly recognized as a biomarker of disease activity in various neurological diseases. The development of a bead-based single molecule array (Simoa) assay for the neurofilament light chain provided a substantial improvement in sensitivity compared with conventional ELISA or electrochemiluminescence-based assays allowing reliable quantification in blood samples.

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Authors and Affiliations

  1. Neurology Clinic and Policlinic, MS Center and Research Center for Clinical Neuroimmunology and Neuroscience Basel (RC2NB), University Hospital Basel, University of Basel, Basel, Switzerland

    Christian Barro, Sarah Storz, Svenya Gröbke, Zuzanna Michalak & Jens Kuhle

Authors
  1. Christian Barro
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  2. Sarah Storz
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  3. Svenya Gröbke
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  4. Zuzanna Michalak
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  5. Jens Kuhle
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Corresponding author

Correspondence to Jens Kuhle .

Editor information

Editors and Affiliations

  1. Neurochemistry Laboratory, Department of Clinical Chemistry, Amsterdam Neuroscience, Amsterdam University Medical Centre, Vrije Universiteit, Amsterdam, The Netherlands

    Charlotte E. Teunissen

  2. Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy at the University of Gothernburg, Mölndal, Sweden

    Henrik Zetterberg

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Barro, C., Storz, S., Gröbke, S., Michalak, Z., Kuhle, J. (2021). Quantification of the Neurofilament Light Chain Protein by Single Molecule Array (Simoa) Assay. In: Teunissen, C.E., Zetterberg, H. (eds) Cerebrospinal Fluid Biomarkers. Neuromethods, vol 168. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1319-1_13

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  • DOI: https://doi.org/10.1007/978-1-0716-1319-1_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1318-4

  • Online ISBN: 978-1-0716-1319-1

  • eBook Packages: Springer Protocols

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