Abstract
The production of doubled haploids (DHs) has proved to be a highly valuable tool to obtain new cultivars. Among the cereals, barley (Hordeum vulgare L.) is the most successful species in large-scale haploid production. Techniques employed for this purpose are based on either the gynogenetic or the androgenetic pathway. Interspecific cross with Hordeum bulbosum L., haploid gene inducer (the hap gene), ovary culture, anther culture (AC), and isolated microspore culture (IMC) are the most used methods. Among all of them, IMC is regarded as a particularly effective system owing to the great increase in green plant numbers per spike and also the higher induction of chromosome doubling when compared with other methods. Thus, IMC provides the best way to mass scale production of new varieties.
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References
Davies PA, Morton S (1998) A comparison of barley isolated microspore and anther culture and the influence of cell culture density. Plant Cell Rep 17:206–210
Kasha KH, Simion E, Oro R, Yao QA, Hu TC, Carlson AR (2001) An improved in vitro technique for isolated microspore culture of barley. Euphytica 120:379–385
Li H, Devaux P (2005) Isolated microspore culture over performs anther culture for green plant regeneration in barley (Hordeum vulgare L.). Acta Physiol Plant 27:611–619
Huang B, Sunderland N (1982) Temperature stress pretreatment in barley anther culture. Ann Bot 49:77–88
Roberts-Oehlschlager SL, Dunwell JM (1990) Barley anther culture: pretreatment on mannitol stimulates production of microspore-derived embryos. Plant Cell Tissue Organ Cult 20:235–240
Cistué L, Ramos A, Castillo AM, Romagosa I (1994) Production of large number of doubled haploid plants from barley anthers pretreated with high concentrations of mannitol. Plant Cell Rep 13:709–712
Echávarri B, Cistué L (2016) Enhancement in androgenesis efficiency in barley (Hordeum vulgare L.) and bread wheat (Triticum aestivum L.) by the addition of dimethyl sulfoxide to the mannitol pretreatment medium. Plant Cell Tissue Organ Cult 125:11–22
Hunter CP (1988) Plant regeneration from microspores of barley, Hordeum vulgare. PhD thesis. Wye College, University of London, London
Mordhorst AP, Lörz H (1993) Embryogenesis and development of isolated barley (Hordeum vulgare L.) microspores are influenced by amount and composition of nitrogen sources in culture media. J Plant Physiol 142:485–492
Olsen FL (1987) Induction of microspore organogenesis in cultured anthers of Hordeum vulgare. The effects of ammonium nitrate, glutamine and asparagine as nitrogen sources. Carlsb Res Commun 52:393–404
Olsen FL (1991) Isolation and cultivation of embryogenic microspores from barley (Hordeum vulgare L.). Hereditas 115:255–266
Kao KN (1981) Plant formation from barley anther cultures with Ficoll media. Z Pflanzenphysiol 103:437–443
Kuhlmann U, Foroughi-Wehr B (1989) Production of doubled haploid lines in frequencies sufficient for barley breeding programs. Plant Cell Rep 8:78–81
Devaux P, Kasha KJ (2009) Overview of barley doubled haploid production. In: Touraev A et al (eds) Advances in haploid production in higher plants. Springer, New York
Nuutila AM, Hämäläinen J, Mannonen L (2000) Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.). Plant Sci 151(1):85–92
Wojnarowiez G, Jacquard C, Devaux P, Sangwan RS, Clément C (2002) Influence of copper sulfate on anther culture in barley (Hordeum vulgare L.). Plant Sci 162(5):843–847
Luckett DJ, Smithard RA (1995) A comparison of several published methods for barley anther culture. Plant Cell Rep 14:763–767
Weyen J (2009) Barley and wheat doubled haploids in breeding. In: Touraev A et al (eds) Advances in haploid production in higher plants. Springer, New York
Davies PA (2003) Barley isolated microspore culture (IMC) method. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. Springer, Dordrecht, pp 49–52
Lu R, Wang Y, Sun Y, Shan L, Chen P, Huang J (2008) Improvement of isolated microspore culture of barley (Hordeum vulgare L.): the effect of floret co-culture. Plant Cell Tissue Organ Cult 93:21–27
Torp AM, Andersen SB (2009) Albinism in microspore culture. In: Touraev A et al (eds) Advances in haploid production in higher plants. Springer, New York
Cistué L, Ramos A, Castillo AM (1999) Influence of anther pretreatment and culture medium composition on the production of barley doubled haploids from model and low responding cultivars. Plant Cell Tissue Organ Cult 55:159–166
Marchand S, Fonquerne G, Clermont I, Laroche L, Huynh TT, Belzile FJ (2008) Androgenic response of barley accessions and F1s with Fusarium head blight resistance. Plant Cell Rep 27:443–445
Cistué L, Ziauddin A, Simion E, Kasha KJ (1995) Effects of culture conditions on isolated microspore response of barley cultivar Igri. Plant Cell Tissue Organ Cult 42:163–169
Kasha KJ, Simion E, Oro R, Shim YS (2003) Barley isolated microspore culture protocol. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. Springer, Dordrecht, pp 43–47
Esteves P, Belzile FJ (2019) Isolated microspore culture in barley. Methods Mol Biol 1900:53–71
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15(3):473–497
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Cistué, L., Echávarri, B. (2021). Barley Isolated Microspore Culture. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2287. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1315-3_8
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DOI: https://doi.org/10.1007/978-1-0716-1315-3_8
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