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Oat Doubled Haploid Production Through Wide Hybridization with Maize

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Part of the Methods in Molecular Biology book series (MIMB,volume 2287)

Abstract

Wide hybridization is one of the haploid-inducing techniques that can accelerate the breeding process. Obtaining new cultivars is crucial to solve the problem of the constantly growing world population and global increase in demand for food, feed and renewable energy under changing environmental conditions. Here, we present a detailed protocol for obtaining oat (Avena sativa L.) doubled haploids (DHs) by pollination with maize (Zea mays L.). After fertilization, not only oat homozygotes, but also oat × maize hybrid zygotes can be formed, and during early embryo development, maize chromosomes are preferentially eliminated, which ultimately results in haploid plant formation. This chapter describes a method to produce oat DHs by crossing oat with maize, covering all steps from crossings to haploid plant regeneration and chromosome doubling.

Key words

  • Avena sativa L.
  • Doubled haploids
  • Wide crossing
  • Zea mays L.

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  • DOI: 10.1007/978-1-0716-1315-3_18
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Acknowledgements

The work was supported by the National Centre for Research and Development, grant No. 12002904/2008 and no PBS3/B8/17/2015, and the Ministry of Agriculture and Rural Development, grant no HORhn-801-1/13.

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Correspondence to Edyta Skrzypek .

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Skrzypek, E., Warchoł, M., Czyczyło-Mysza, I., Juzoń, K., Dziurka, K., Marcińska, I. (2021). Oat Doubled Haploid Production Through Wide Hybridization with Maize. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2287. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1315-3_18

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  • DOI: https://doi.org/10.1007/978-1-0716-1315-3_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1314-6

  • Online ISBN: 978-1-0716-1315-3

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