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Oat (Avena sativa L.) Anther Culture

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Part of the Methods in Molecular Biology book series (MIMB,volume 2287)


Production of doubled haploids (DHs) by androgenesis is a promising and convenient alternative to traditionally used breeding techniques. Low response of anther culture and strong genotype dependency in the development of embryo-like structures (ELS) was reported for oat (Avena sativa L.). Total homozygosity has been reached in one generation. This chapter describes a step-by-step protocol that can be useful for androgenesis studies and oat DH line production through anther culture.

Key words

  • Androgenesis
  • Avena sativa L.
  • Doubled haploids
  • Haploids
  • Pretreatment

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  • DOI: 10.1007/978-1-0716-1315-3_17
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The project was supported by the Ministry of Agriculture and Rural Development, grant No. HORhn–4040dec13/08 and the Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences.

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Correspondence to Marzena Warchoł .

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Warchoł, M., Dziurka, K., Czyczyło-Mysza, I., Juzoń, K., Marcińska, I., Skrzypek, E. (2021). Oat (Avena sativa L.) Anther Culture. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2287. Humana, New York, NY.

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  • Print ISBN: 978-1-0716-1314-6

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