Skip to main content

Oat (Avena sativa L.) Anther Culture

  • 503 Accesses

Part of the Methods in Molecular Biology book series (MIMB,volume 2287)

Abstract

Production of doubled haploids (DHs) by androgenesis is a promising and convenient alternative to traditionally used breeding techniques. Low response of anther culture and strong genotype dependency in the development of embryo-like structures (ELS) was reported for oat (Avena sativa L.). Total homozygosity has been reached in one generation. This chapter describes a step-by-step protocol that can be useful for androgenesis studies and oat DH line production through anther culture.

Key words

  • Androgenesis
  • Avena sativa L.
  • Doubled haploids
  • Haploids
  • Pretreatment

This is a preview of subscription content, access via your institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-1-0716-1315-3_17
  • Chapter length: 10 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   139.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-1315-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   139.99
Price excludes VAT (USA)
Hardcover Book
USD   179.99
Price excludes VAT (USA)
Fig. 1

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Rines HW (1983) Oat anther culture: genotype effects on callus initiation and the production of haploid plant. Crop Sci 23:268–227

    CrossRef  Google Scholar 

  2. Kiviharju E, Pehu E (1998) The effect of cold and heat pretreatments on anther culture response of Avena sativa and A. sterilis. Plant Cell Tiss Org Cult 54:97–104

    CrossRef  Google Scholar 

  3. Kiviharju E, Moisander S, Laurila J (2005) Improved green plant regeneration rates from oat anther culture and the agronomic performance of some DH lines. Plant Cell Tissue Organ Cult 81:1–9

    CAS  CrossRef  Google Scholar 

  4. Kiviharju E, Moisander S, Tanhuanpää P (2017) Oat anther culture and use of DH-lines for genetic mapping. In: Gasparis S (ed) Oat. Methods in molecular biology, vol 1536. Humana Press, New York, pp 71–93

    Google Scholar 

  5. Warchoł M, Czyczyło-Mysza I, Marcińska I, Dziurka K, Noga A, Kapłoniak K, Pilipowicz M, Skrzypek E (2019) Factors inducing regeneration response in oat (Avena sativa L.) anther culture. In Vitro Cell Dev Biol Plant 55(5):595–604

    CrossRef  Google Scholar 

  6. Ferrie AMR, Irmen KI, Beattie AD, Rossnagel BG (2014) Isolated microspore culture of oat (Avena sativa L.) for the production of doubled haploids: effect of pre-culture and post-culture conditions. Plant Cell Tissue Organ Cult 116:89–96

    CAS  CrossRef  Google Scholar 

  7. Sood S, Dwivedi S (2015) Doubled haploid platform: an accelerated breeding approach for crop improvement. In: Bahadur B, Venkat Rajam M, Sahijram L, Krishnamurthy K (eds) Plant biology and biotechnology. Springer, New Delhi, pp 89–111

    CrossRef  Google Scholar 

  8. Hoagland DR, Arnon DI (1938) A water culture method for growing plants without soil. Circ Univ Calif, Agric Exp Stn No 347

    Google Scholar 

  9. Ouyang TW, Jia SE, Zhang C, Chen X, Feng G (1989) A new synthetic medium (W14) for wheat anther culture. Annual report 1987–1988. Institute of Genetics Academia Sinica, Beijing, pp 91–92

    Google Scholar 

  10. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    CAS  CrossRef  Google Scholar 

  11. Sambrook J, Fritschi EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

Download references

Acknowledgments

The project was supported by the Ministry of Agriculture and Rural Development, grant No. HORhn–4040dec13/08 and the Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Marzena Warchoł .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 2021 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Verify currency and authenticity via CrossMark

Cite this protocol

Warchoł, M., Dziurka, K., Czyczyło-Mysza, I., Juzoń, K., Marcińska, I., Skrzypek, E. (2021). Oat (Avena sativa L.) Anther Culture. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2287. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1315-3_17

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-1315-3_17

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1314-6

  • Online ISBN: 978-1-0716-1315-3

  • eBook Packages: Springer Protocols