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Production of Wheat Doubled Haploids Through Intergeneric Hybridization with Maize

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Doubled Haploid Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2287))

Abstract

The intergeneric hybridization of wheat (Triticum aestivum L.) with maize (Zea mays L.) enables the production of doubled haploids (DHs) of wheat from all wheat hybrids with high efficiencies. Wheat and maize donor plants are raised in environmentally controlled greenhouses until crossing. Before anthesis, wheat spikes are emasculated and then pollinated with maize. Auxin is applied to each individual wheat floret 1 day after pollination. About 2 weeks after crossing, in vitro embryo culture is performed, enabling the regeneration of haploid wheat plantlets after maize chromosome elimination. Haploid plantlets are transferred to the greenhouse and after recovery, their genome is doubled with colchicine. Haploid plantlets can be sampled for DNA extractions and molecular analyses to aid the rapid discard of undesirable plantlets. Doubled haploid plants are raised in a greenhouse until maturity. Seeds of each fertile DH are harvested and often sown the same year. Several cycles of multiplication and evaluation in replicated plot trials and different geographical locations are then done to select the best candidate(s) for varietal registration.

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References

  1. Kasha KJ, Reinbergs E (1975) Utilization of haploidy in barley. In: Gaul H (ed) Barley genetics III, proc 3rd Int barley genet Symp, Garching. Springer, Berlin, pp 307–315

    Google Scholar 

  2. Pickering RA, Devaux P (1992) Haploid production: approaches and use in plant breeding. In: Shewry PR (ed) Barley: genetics, Molecular Biology and Biotechnology. CAB Int Publ, Wallingford, pp 511–539

    Google Scholar 

  3. Forster BP, Thomas WTB (2005) Doubled haploids in genetics and plant breeding. In: Janick J (ed) Plant breeding reviews, vol 25. John Wiley & Sons, New York, pp 57–88

    Google Scholar 

  4. Palmer CE, Keller WA (2005) Overview of haploid. In: Palmer CE, Keller WA, Kasha KJ (eds) Biotechnology in agriculture and forestry, Vol 56. Haploids in crop improvement II. Springer-Verlag, Berlin, Heidelberg, pp 3–9

    Google Scholar 

  5. Laurie DA, Bennett MD (1988) The production of haploid wheat plants from wheat x maize crosses. Theor Appl Genet 76:393–397

    Article  CAS  Google Scholar 

  6. Devaux P, Cistué L (2016) Wheat doubled haploids: production to sequencing – what makes them so appealing? In: Bonjean A, Angus WJ, Ginkel van M (eds) The world wheat book, a history of wheat breeding, vol 3. Lavoisier Tec&doc, Cachan Cedex, pp 885–938

    Google Scholar 

  7. Gamborg OL, Miller RA, Olima K (1968) Nutrient requirements of suspension cultures of soybean root cell. Exp Cell Res 50:151–158

    Article  CAS  Google Scholar 

  8. Lefebvre D, Devaux P (1996) Doubled haploids of wheat from wheat x maize crosses: genotypic influence, fertility and inheritance of the 1BL-1RS chromosome. Theor Appl Genet 93:1267–1273

    Article  CAS  Google Scholar 

  9. Laurie DA, Reymondie S (1991) High frequencies of fertilization and haploid seedling production in crosses between commercial hexaploidy wheat varieties and maize. Plant Breed 106:182–189

    Article  Google Scholar 

  10. Knox RE, Clarke JM, DePauw RM (2000) Dicamba and growth condition effects on doubled haploid production in durum wheat crossed with maize. Plant Breed 119:289–298

    Article  Google Scholar 

  11. Niu Z, Jiang A, Hammad WA, Oladzadabbasabadi A, Xu SS, Mergoum M, Elias EM (2014) Review of doubled haploid production in durum and common wheat through wheat x maize hybridization. Plant Breed 133:313–320

    Article  CAS  Google Scholar 

  12. Bains NS, Mangat GS, Singh K, Nanda GS (1998) A simple technique for the identification of embryo-carrying seeds from wheat x maize crosses prior to dissection. Plant Breed 117:191–192

    Article  Google Scholar 

  13. Pickering RA (1980) Use of the doubled haploid technique in barley breeding at the welsh plant breeding station. Rep Welsh Pl Breed Stn 1979:208–226

    Google Scholar 

  14. Broughton S, Castello M, Liu L, Killen J, Hepworth A, O’Leary R (2020) The effect of caffeine and trifluralin on chromosome doubling in wheat anther culture. Plants 9:105. https://doi.org/10.3390/plants9010105

    Google Scholar 

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Acknowledgments

The author gratefully acknowledges contributions of colleagues and technical support to the development over the years of the described protocol. Many thanks are due to Dr. R.A. Pickering for critically reviewing this manuscript. This article is dedicated to the memory of Mr. Michel Desprez, breeder and scientist, former Research Director at Florimond Desprez.

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Correspondence to Pierre Devaux .

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Devaux, P. (2021). Production of Wheat Doubled Haploids Through Intergeneric Hybridization with Maize. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2287. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1315-3_14

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  • DOI: https://doi.org/10.1007/978-1-0716-1315-3_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1314-6

  • Online ISBN: 978-1-0716-1315-3

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