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Helicobacter Pylori pp 175–190Cite as

Evaluating Host Responses to Helicobacter pylori Using ELISA and Western Blot

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Part of the Methods in Molecular Biology book series (MIMB,volume 2283)

Abstract

Western blot and enzyme-linked immunosorbent assay (ELISA) are antibody-mediated techniques which are widely used for the detection and characterization of alterations in host protein expression following H. pylori infection . Both techniques are highly specific and sensitive for protein detection, with Western blot detection sensitivity as low as picogram amounts of the protein of interest, while the typical ELISA detection range is 0.01–0.1 ng. Here we provide an experimental example to demonstrate the application of these techniques for the determination of macrophage inflammatory responses following H. pylori infection .

Key words

  • Protein expression
  • ELISA
  • Western
  • Inflammatory cytokines
  • Innate Immune response

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Acknowledgments

This study was supported by funding from the Irish Research Council (GOIPG 2018/3047 to C.N.); a TCD John Scott Fellowship to G.B.; and EU Horizon 2020 Research and Innovation Programme (Marie Sklodowska-Curie grant agreement No 721906 to E.F.).

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Authors and Affiliations

  1. School of Biochemistry & Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland

    Ciara Nulty, Gillian Barber, Ewelina Flis & Emma M. Creagh

Authors
  1. Ciara Nulty
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  2. Gillian Barber
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  3. Ewelina Flis
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  4. Emma M. Creagh
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Corresponding author

Correspondence to Emma M. Creagh .

Editor information

Editors and Affiliations

  1. Department of Clinical Medicine, School of Medicine, Trinity College Dublin, Dublin 2, Ireland

    Dr. Sinead M Smith

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Nulty, C., Barber, G., Flis, E., Creagh, E.M. (2021). Evaluating Host Responses to Helicobacter pylori Using ELISA and Western Blot. In: Smith, S.M. (eds) Helicobacter Pylori. Methods in Molecular Biology, vol 2283. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1302-3_16

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  • DOI: https://doi.org/10.1007/978-1-0716-1302-3_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1301-6

  • Online ISBN: 978-1-0716-1302-3

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