Lung Cancer pp 175-186 | Cite as
A Method for the Establishment and Characterization of Mouse Lung Adenocarcinoma Cell Lines that Mimic Traits of Human Adenocarcinomas
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Abstract
Lung adenocarcinoma (LADC) is the leading cause of cancer death worldwide and is largely inflicted by carcinogens contained in tobacco smoke. The generation of cell lines mimicking traits of human LADC will profoundly advance our understanding of the pathobiology of the disease, as they offer an easy and valuable tool to study the cellular and molecular aspects of carcinogenesis. Here we describe a detailed protocol for the generation of such cell lines, following the exposure of experimental mouse strains to different tobacco carcinogens and isolation of the resulting lung tumors.
Key words
Lung adenocarcinoma Smoke-induced carcinogenesis Tobacco smoke Mouse cell lines Chemical carcinogenesisNotes
Acknowledgments
The work was supported by European Research Council 2010 Starting Independent Investigator and 2015 Proof of Concept Grants (grant numbers 260524 and 679345 respectively; to G.T.S.).
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