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Cell Culture Assays

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Abstract

Cell culture assays play an important role in drug research along with animal tests with the principle of ‘3Rs’—Reduction, Refinement and Replacement—due to its quick and easy way of drug response analysis, as well as high throughput in assays, simplicity and cost-effectiveness. This chapter explains various procedures, cell viability, cytotoxicity and inflammatory response, for the evaluation of cytotoxicity of novel compounds or extracts. The protocols like propidium iodide used cell cycle assay and apoptosis assay, Alamar Blue method in Caco-2 cells, cell viability test in Caco-2 cells using lipopolysaccharide (LPS) and cytotoxicity against RAW 264.7 for the determination of anti-inflammatory activity—together with the assays for nitric oxide (NO) determination and scratch assay for testing the proliferation of fibroblast cells and inflammatory response in AGS cells model—elucidate the detailed and standardized protocols for the in vitro assays with cell lines.

Key words

  • Alamar Blue
  • NO
  • LPS
  • AGS
  • Scratch assay
  • Fibroblast cells
  • Propidium iodide
  • Annexin V

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  • DOI: 10.1007/978-1-0716-1233-0_2
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Arunachalam, K., Sasidharan, S.P. (2021). Cell Culture Assays. In: Bioassays in Experimental and Preclinical Pharmacology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1233-0_2

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  • DOI: https://doi.org/10.1007/978-1-0716-1233-0_2

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1232-3

  • Online ISBN: 978-1-0716-1233-0

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