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Melanoma pp 213–222Cite as

Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells

Part of the Methods in Molecular Biology book series (MIMB,volume 2265)

Abstract

Within the last decade, circulating tumor cells (CTCs) have emerged as a promising biomarker for prognostication, treatment monitoring, and detection of markers of treatment resistance, and their isolation can be used as a minimally invasive means of profiling tumors across multiple body sites. However, CTCs represent a minuscule fraction of the total circulating cells in a patient. Therefore, sensitive isolation methods are needed for the detection and downstream analysis of these cells. Herein we describe a sensitive method for melanoma CTC isolation using a multi-marker immunomagnetic bead method. This method has been purposely optimized to detect CTCs in melanoma patients.

Key words

  • Circulating tumor cells (CTCs )
  • Immunomagnetic beads
  • Multi-marker

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Acknowledgments

A.B.B. and E.A. are supported by an Edith Cowan University Postgraduate Scholarship. A.B.B. is supported by a Cancer Council of Western Australia PhD Top-up Scholarship. E.S.G. holds a fellowship from the Cancer Council Western Australia.

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Authors and Affiliations

  1. School of Medical and Health Sciences, Edith Cowan University, Joondalup, WA, Australia

    Aaron B. Beasley, Emmanuel Acheampong, Weitao Lin & Elin S. Gray

Authors
  1. Aaron B. Beasley
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  2. Emmanuel Acheampong
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  3. Weitao Lin
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  4. Elin S. Gray
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Corresponding author

Correspondence to Elin S. Gray .

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Editors and Affiliations

  1. Department of Biology, Hampden-Sydney College, Hampden-Sydney, VA, USA

    Ph.D. Kristian M. Hargadon

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Beasley, A.B., Acheampong, E., Lin, W., Gray, E.S. (2021). Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells. In: Hargadon, K.M. (eds) Melanoma. Methods in Molecular Biology, vol 2265. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1205-7_16

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  • DOI: https://doi.org/10.1007/978-1-0716-1205-7_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1204-0

  • Online ISBN: 978-1-0716-1205-7

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