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Protein Assembly: Defining the Strength of Protein-Protein Interactions Coupling the Theory with Experiments

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Allostery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2253))

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Abstract

In this paper we report a procedure to analyze protein homodimer interfaces.

We approached the problem by means of a topological methodology. In particular, we analyzed the subunits interface of about 50 homodimers and we have defined a few parameters that allow to organize these proteins in six different classes. The main characteristics of each class of homodimers have been discussed also taking into account their stabilization energy, as reported in the literature from the experimental measurements. A paradigmatic example for each class has been reported and a graphical representation proposed in order to better explain the meaning of the parameters chosen.

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Authors and Affiliations

  1. Department of Experimental Medicine and Surgery, University of Rome “Tor Vergata”, Rome, Italy

    Giampiero Mei, Almerinda Di Venere & Alessandro Finazzi Agrò

  2. Unit of Chemical-Physics Fundamentals in Chemical Engineering, Department of Engineering, Università Campus Bio-Medico di Rome, Rome, Italy

    Luisa Di Paola

Authors
  1. Giampiero Mei
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  2. Almerinda Di Venere
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  3. Luisa Di Paola
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  4. Alessandro Finazzi Agrò
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Corresponding author

Correspondence to Giampiero Mei .

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Editors and Affiliations

  1. Unit of Chemical-Physics Fundamentals in Chemical Engineering Department of Engineering, Universitá Campus Bio-Medico di Rome, Rome, Italy

    Luisa Di Paola

  2. Environment and Health Department, Instituto Superiore di Sanità, Rome, Italy

    Alessandro Giuliani

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Mei, G., Di Venere, A., Di Paola, L., Finazzi Agrò, A. (2021). Protein Assembly: Defining the Strength of Protein-Protein Interactions Coupling the Theory with Experiments. In: Di Paola, L., Giuliani, A. (eds) Allostery. Methods in Molecular Biology, vol 2253. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1154-8_6

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  • DOI: https://doi.org/10.1007/978-1-0716-1154-8_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1153-1

  • Online ISBN: 978-1-0716-1154-8

  • eBook Packages: Springer Protocols

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