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Determination of TE Insertion Positions Using Transposon Display

Part of the Methods in Molecular Biology book series (MIMB,volume 2250)

Abstract

Mapping the genomic location to which transposons jumped is of greatest interest to transposon biologists. Transposon display (TD) is the technique of choice that is easy and fast in determining the neo-insertion positions of a target transposon. Essentially, tagging of transposon is performed by digesting genomic DNA, ligating adaptors to digested DNA ends and PCR amplifying genomic regions flanking the transposon of interest. In this chapter, the experimental procedure of TD is described using Onsen retrotransposon of Arabidopsis as an example.

Key words

  • Transposon display
  • Retrotransposon
  • Transposition
  • Onsen
  • Arabidopsis

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  • DOI: 10.1007/978-1-0716-1134-0_11
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Acknowledgments

This work was supported by the Strategic Priority Research Program of Chinese Academy of Sciences (XDB27030209) and the National Natural Science Foundation of China (31970518) granted to J.C.

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Correspondence to Jungnam Cho .

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Kim, E.Y., Fan, W., Cho, J. (2021). Determination of TE Insertion Positions Using Transposon Display. In: Cho, J. (eds) Plant Transposable Elements. Methods in Molecular Biology, vol 2250. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1134-0_11

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  • DOI: https://doi.org/10.1007/978-1-0716-1134-0_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1133-3

  • Online ISBN: 978-1-0716-1134-0

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